Abstract
Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 99-105 |
| Number of pages | 7 |
| Journal | Virology |
| Volume | 335 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 25 2005 |
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Keywords
- Envelope protein
- Flavivirus
- Neutralizing epitope
- West Nile virus
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains. / Li, Li; Barrett, Alan; Beasley, David.
In: Virology, Vol. 335, No. 1, 25.04.2005, p. 99-105.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains
AU - Li, Li
AU - Barrett, Alan
AU - Beasley, David
PY - 2005/4/25
Y1 - 2005/4/25
N2 - Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.
AB - Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.
KW - Envelope protein
KW - Flavivirus
KW - Neutralizing epitope
KW - West Nile virus
UR - http://www.scopus.com/inward/record.url?scp=16844382354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16844382354&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2005.02.011
DO - 10.1016/j.virol.2005.02.011
M3 - Article
VL - 335
SP - 99
EP - 105
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -