Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains

Li Li; Alan D.T. Barrett; David W.C. Beasley

doi:10.1016/j.virol.2005.02.011

Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains

Li Li, Alan D.T. Barrett, David W.C. Beasley

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.

Original language	English (US)
Pages (from-to)	99-105
Number of pages	7
Journal	Virology
Volume	335
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2005.02.011
State	Published - Apr 25 2005

Keywords

Envelope protein
Flavivirus
Neutralizing epitope
West Nile virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2005.02.011

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title = "Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains",

abstract = "Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.",

keywords = "Envelope protein, Flavivirus, Neutralizing epitope, West Nile virus",

author = "Li Li and Barrett, {Alan D.T.} and Beasley, {David W.C.}",

note = "Funding Information: This study was supported in part by grants from the Centers for Disease Control and Prevention and the State of Texas Advanced Research Program. DB is supported by the James W. McLaughlin Fellowship Fund.",

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AU - Li, Li

AU - Barrett, Alan D.T.

AU - Beasley, David W.C.

N1 - Funding Information: This study was supported in part by grants from the Centers for Disease Control and Prevention and the State of Texas Advanced Research Program. DB is supported by the James W. McLaughlin Fellowship Fund.

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N2 - Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.

AB - Neutralization of flaviviruses by antibody is primarily mediated via epitopes in the viral envelope (E) protein. Comparative studies using neutralizing monoclonal antibodies revealed differential expression of epitopes within the E protein domain III of ten naturally occurring West Nile virus strains representing major subtypes of genetic lineages 1 and 2. Residues that defined these subtype-specific determinants were identified by mutational studies and found to be surface exposed in the domain III structure. Mutations of residue 332 had the most significant effects on variation of domain III neutralizing epitopes among strains.

KW - Envelope protein

KW - Flavivirus

KW - Neutralizing epitope

KW - West Nile virus

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Differential expression of domain III neutralizing epitopes on the envelope proteins of West Nile virus strains

Abstract

Keywords

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