Differential expression of immediate early genes in distinct layers of rat cerebral cortex after selective immunolesion of the forebrain cholinergic system

S. Rossner, J. R. Perez-Polo, R. G. Wiley, R. Schliebs, V. Bigl

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    The aim of this study was to show whether reduction or loss of cortical cholinergic activity results in any particular change in the expression of the proto-oncogenes c-fos and/or c-jun. To produce cortical cholinergic hypofunction, the monoclonal antibody, 192IgG, to the low-affinity nerve growth factor receptor p75(NGFR) coupled to a cytotoxin, saporin, was used as an efficient and selective immunotoxin for cholinergic neurons in rat basal forebrain. Brain sections of adult rats that had received an intracerebroventricular injection of 4 μg of the 192IgG-saporin were subjected to in situ hybridization using oligonucleotides to detect c-fos and c-jun mRNA. Autoradiographs obtained were evaluated by quantitative image analysis. Seven days following injection of the immunotoxin there was a dramatic loss in acetylcholinesterase staining in frontal, parietal, piriform, temporal, and occipital cortices, hippocampus, and olfactory bulb, but not in the striatum and cerebellum. In situ hybridization revealed a considerable increase in the level of c-fos mRNA in the lateral septum following the cholinergic lesion, whereas in the medial septum both c-fos and c-jun mRNA were elevated. Immunolesioning led to a distinct and specific increase in the level of c-jun but not c-fos mRNA in the parietal and occipital cortex that was restricted to cortical layer IV. These data suggest that reduced cortical cholinergic activity differentially regulates expression of c-fos/c-jun genes in distinct cortical regions of the rat brain.

    Original languageEnglish (US)
    Pages (from-to)282-293
    Number of pages12
    JournalJournal of Neuroscience Research
    Volume38
    Issue number3
    DOIs
    StatePublished - 1994

    Fingerprint

    Immediate-Early Genes
    Prosencephalon
    Cerebral Cortex
    Cholinergic Agents
    Immunotoxins
    Nerve Growth Factor Receptor
    Occipital Lobe
    Messenger RNA
    Parietal Lobe
    In Situ Hybridization
    jun Genes
    fos Genes
    Injections
    Cholinergic Neurons
    Olfactory Bulb
    Cytotoxins
    Brain
    Frontal Lobe
    Temporal Lobe
    Acetylcholinesterase

    Keywords

    • 192IgG-saporin
    • acetylcholinesterase
    • c-fos
    • c-jun
    • image analysis
    • in situ hybridization

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Differential expression of immediate early genes in distinct layers of rat cerebral cortex after selective immunolesion of the forebrain cholinergic system. / Rossner, S.; Perez-Polo, J. R.; Wiley, R. G.; Schliebs, R.; Bigl, V.

    In: Journal of Neuroscience Research, Vol. 38, No. 3, 1994, p. 282-293.

    Research output: Contribution to journalArticle

    @article{e43101496e3c42a99e284cbb9c8eeef3,
    title = "Differential expression of immediate early genes in distinct layers of rat cerebral cortex after selective immunolesion of the forebrain cholinergic system",
    abstract = "The aim of this study was to show whether reduction or loss of cortical cholinergic activity results in any particular change in the expression of the proto-oncogenes c-fos and/or c-jun. To produce cortical cholinergic hypofunction, the monoclonal antibody, 192IgG, to the low-affinity nerve growth factor receptor p75(NGFR) coupled to a cytotoxin, saporin, was used as an efficient and selective immunotoxin for cholinergic neurons in rat basal forebrain. Brain sections of adult rats that had received an intracerebroventricular injection of 4 μg of the 192IgG-saporin were subjected to in situ hybridization using oligonucleotides to detect c-fos and c-jun mRNA. Autoradiographs obtained were evaluated by quantitative image analysis. Seven days following injection of the immunotoxin there was a dramatic loss in acetylcholinesterase staining in frontal, parietal, piriform, temporal, and occipital cortices, hippocampus, and olfactory bulb, but not in the striatum and cerebellum. In situ hybridization revealed a considerable increase in the level of c-fos mRNA in the lateral septum following the cholinergic lesion, whereas in the medial septum both c-fos and c-jun mRNA were elevated. Immunolesioning led to a distinct and specific increase in the level of c-jun but not c-fos mRNA in the parietal and occipital cortex that was restricted to cortical layer IV. These data suggest that reduced cortical cholinergic activity differentially regulates expression of c-fos/c-jun genes in distinct cortical regions of the rat brain.",
    keywords = "192IgG-saporin, acetylcholinesterase, c-fos, c-jun, image analysis, in situ hybridization",
    author = "S. Rossner and Perez-Polo, {J. R.} and Wiley, {R. G.} and R. Schliebs and V. Bigl",
    year = "1994",
    doi = "10.1002/jnr.490380306",
    language = "English (US)",
    volume = "38",
    pages = "282--293",
    journal = "Journal of Neuroscience Research",
    issn = "0360-4012",
    publisher = "Wiley-Liss Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - Differential expression of immediate early genes in distinct layers of rat cerebral cortex after selective immunolesion of the forebrain cholinergic system

    AU - Rossner, S.

    AU - Perez-Polo, J. R.

    AU - Wiley, R. G.

    AU - Schliebs, R.

    AU - Bigl, V.

    PY - 1994

    Y1 - 1994

    N2 - The aim of this study was to show whether reduction or loss of cortical cholinergic activity results in any particular change in the expression of the proto-oncogenes c-fos and/or c-jun. To produce cortical cholinergic hypofunction, the monoclonal antibody, 192IgG, to the low-affinity nerve growth factor receptor p75(NGFR) coupled to a cytotoxin, saporin, was used as an efficient and selective immunotoxin for cholinergic neurons in rat basal forebrain. Brain sections of adult rats that had received an intracerebroventricular injection of 4 μg of the 192IgG-saporin were subjected to in situ hybridization using oligonucleotides to detect c-fos and c-jun mRNA. Autoradiographs obtained were evaluated by quantitative image analysis. Seven days following injection of the immunotoxin there was a dramatic loss in acetylcholinesterase staining in frontal, parietal, piriform, temporal, and occipital cortices, hippocampus, and olfactory bulb, but not in the striatum and cerebellum. In situ hybridization revealed a considerable increase in the level of c-fos mRNA in the lateral septum following the cholinergic lesion, whereas in the medial septum both c-fos and c-jun mRNA were elevated. Immunolesioning led to a distinct and specific increase in the level of c-jun but not c-fos mRNA in the parietal and occipital cortex that was restricted to cortical layer IV. These data suggest that reduced cortical cholinergic activity differentially regulates expression of c-fos/c-jun genes in distinct cortical regions of the rat brain.

    AB - The aim of this study was to show whether reduction or loss of cortical cholinergic activity results in any particular change in the expression of the proto-oncogenes c-fos and/or c-jun. To produce cortical cholinergic hypofunction, the monoclonal antibody, 192IgG, to the low-affinity nerve growth factor receptor p75(NGFR) coupled to a cytotoxin, saporin, was used as an efficient and selective immunotoxin for cholinergic neurons in rat basal forebrain. Brain sections of adult rats that had received an intracerebroventricular injection of 4 μg of the 192IgG-saporin were subjected to in situ hybridization using oligonucleotides to detect c-fos and c-jun mRNA. Autoradiographs obtained were evaluated by quantitative image analysis. Seven days following injection of the immunotoxin there was a dramatic loss in acetylcholinesterase staining in frontal, parietal, piriform, temporal, and occipital cortices, hippocampus, and olfactory bulb, but not in the striatum and cerebellum. In situ hybridization revealed a considerable increase in the level of c-fos mRNA in the lateral septum following the cholinergic lesion, whereas in the medial septum both c-fos and c-jun mRNA were elevated. Immunolesioning led to a distinct and specific increase in the level of c-jun but not c-fos mRNA in the parietal and occipital cortex that was restricted to cortical layer IV. These data suggest that reduced cortical cholinergic activity differentially regulates expression of c-fos/c-jun genes in distinct cortical regions of the rat brain.

    KW - 192IgG-saporin

    KW - acetylcholinesterase

    KW - c-fos

    KW - c-jun

    KW - image analysis

    KW - in situ hybridization

    UR - http://www.scopus.com/inward/record.url?scp=0028305165&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028305165&partnerID=8YFLogxK

    U2 - 10.1002/jnr.490380306

    DO - 10.1002/jnr.490380306

    M3 - Article

    C2 - 7932863

    AN - SCOPUS:0028305165

    VL - 38

    SP - 282

    EP - 293

    JO - Journal of Neuroscience Research

    JF - Journal of Neuroscience Research

    SN - 0360-4012

    IS - 3

    ER -