Differential expression of proteins in listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions

Réka Ágoston, Kamlesh Soni, Palmy R. Jesudhasan, William Russell, Csilla Mohácsi-Farkas, Suresh D. Pillai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Listeria monocytogenes is a foodborne pathogen capable of employing stress adaptive responses to evade a variety of stressors including temperature stress. We employed two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight analysis to study the differential expression of L. monocytogenes (ATCC 43256) soluble proteins at heat shock (60°C) conditions, prolonged heat shock (60°C for 9 minutes) conditions, and thermotolerance-inducing (48°C for 30 minutes followed by 60°C for 9 minutes) conditions. We compared the proteome of L. monocytogenes under these conditions to the proteome at 37°C. Eighteen proteins were differentially expressed at 60°C (6 up-regulated and 12 down-regulated), 21 proteins were differentially expressed (12 up-regulated and 9 down-regulated) when the cells were exposed to 60°C for 9 minutes, and 20 proteins were differentially expressed (10 up-regulated and 10 down-regulated) when cells were initially exposed to 48°C for 30 minutes before 60°C for 9 minutes. There was one unidentifiable protein with observed molecular weight of 50?kDa which was differentially expressed across the three temperature treatments. Thermotolerance-inducing conditions caused the up-regulation of a protein by as much as 12-fold. DnaN, a previously identified stress protein, was up-regulated almost threefold at 60°C. TcsA, a lipoprotein (CD4 T cell-stimulating antigen), and Gap (glyceraldehyde-3-phosphate-dehydrogenase) were selectively expressed under prolonged heat shock conditions suggesting their potential as candidate marker proteins targets for identifying temperature-stressed L. monocytogenes cells.

Original languageEnglish (US)
Pages (from-to)1133-1140
Number of pages8
JournalFoodborne Pathogens and Disease
Volume6
Issue number9
DOIs
StatePublished - Nov 1 2009
Externally publishedYes

Fingerprint

Listeria monocytogenes
heat tolerance
heat stress
Shock
protein synthesis
Hot Temperature
Proteins
proteins
Proteome
Heat-Shock Proteins
Temperature
proteome
Glyceraldehyde-3-Phosphate Dehydrogenases
Electrophoresis, Gel, Two-Dimensional
temperature
Lipoproteins
glyceraldehyde-3-phosphate dehydrogenase
Thermotolerance
two-dimensional gel electrophoresis
Lasers

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Differential expression of proteins in listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions. / Ágoston, Réka; Soni, Kamlesh; Jesudhasan, Palmy R.; Russell, William; Mohácsi-Farkas, Csilla; Pillai, Suresh D.

In: Foodborne Pathogens and Disease, Vol. 6, No. 9, 01.11.2009, p. 1133-1140.

Research output: Contribution to journalArticle

Ágoston, Réka ; Soni, Kamlesh ; Jesudhasan, Palmy R. ; Russell, William ; Mohácsi-Farkas, Csilla ; Pillai, Suresh D. / Differential expression of proteins in listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions. In: Foodborne Pathogens and Disease. 2009 ; Vol. 6, No. 9. pp. 1133-1140.
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