TY - JOUR
T1 - Differential expression of the mouse α1-acid glycoprotein genes (AGP-1 and AGP-2) during inflammation and aging
AU - Carter, Kenneth C.
AU - Post, David J.
AU - Papaconstantinou, John
N1 - Funding Information:
We greatly appreciate Marg Hillesheim's assistance during the preparation of this manuscript. This research was supported by a grant from Shriners Burns Institute, Galveston Unit (to John Papaconstantinou).
PY - 1991/6/13
Y1 - 1991/6/13
N2 - In this study we investigated the expression of the Balb/c mouse α1-acid glycoprotein genes. Mice, like humans, have two distinct α1-acid glycoprotein mRNAs. As in humans and rats, mouse α1-acid glycoprotein is a strong acute-phase reactant and its expression can be induced by acute-phase stimulatory agents such as bacterial lipopolysaccharides. Southern analysis and partial sequencing of different α1-acid glycoprotein genomic clones indicated the existence of three distinct α1-acid glycoprotein genes in the Balb/c genome. Using oligonucleotide hybridization, we showed that two of the three genes were expressed while the third gene was either not expressed or expressed at extremely low levels. The mRNA levels for the two expressed genes, α1-acid glycoprotein-1 and α1-acid glycoprotein-2, were both induced during the acute-phase response. However, α1-acid glycoprotein-2 mRNA was present in at least 10-fold higher levels in both induced and uninduced mice. There were also differences in the developmental patterns of the two mRNAs in that the constitutive α1-acid glycoprotein-1 mRNA levels increased 20-fold between 2 and 7 months, while α1-acid glycoprotein-2 mRNA pools remained constant. During the acute-phase response in aged animals, there was an increase in the time required for both mRNAs to respond, and the maximum induced level of both mRNAs decreased. These studies set the stage for future experiments to determine the mechanisms by which the different α1-acid glycoprotein genes are regulated during the acute-phase response and how aging affects these regulatory processes.
AB - In this study we investigated the expression of the Balb/c mouse α1-acid glycoprotein genes. Mice, like humans, have two distinct α1-acid glycoprotein mRNAs. As in humans and rats, mouse α1-acid glycoprotein is a strong acute-phase reactant and its expression can be induced by acute-phase stimulatory agents such as bacterial lipopolysaccharides. Southern analysis and partial sequencing of different α1-acid glycoprotein genomic clones indicated the existence of three distinct α1-acid glycoprotein genes in the Balb/c genome. Using oligonucleotide hybridization, we showed that two of the three genes were expressed while the third gene was either not expressed or expressed at extremely low levels. The mRNA levels for the two expressed genes, α1-acid glycoprotein-1 and α1-acid glycoprotein-2, were both induced during the acute-phase response. However, α1-acid glycoprotein-2 mRNA was present in at least 10-fold higher levels in both induced and uninduced mice. There were also differences in the developmental patterns of the two mRNAs in that the constitutive α1-acid glycoprotein-1 mRNA levels increased 20-fold between 2 and 7 months, while α1-acid glycoprotein-2 mRNA pools remained constant. During the acute-phase response in aged animals, there was an increase in the time required for both mRNAs to respond, and the maximum induced level of both mRNAs decreased. These studies set the stage for future experiments to determine the mechanisms by which the different α1-acid glycoprotein genes are regulated during the acute-phase response and how aging affects these regulatory processes.
KW - Acute phase response
KW - Aging
KW - Gene regulation
KW - mRNA
KW - α-Acid glycoprotein gene
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U2 - 10.1016/0167-4781(91)90008-A
DO - 10.1016/0167-4781(91)90008-A
M3 - Article
C2 - 2054382
AN - SCOPUS:0025903780
SN - 0167-4781
VL - 1089
SP - 197
EP - 205
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 2
ER -