Differential expression of three C/EBP isoforms in multiple tissues during the acute phase response

Tawfiq Alam, Mi Ra An, John Papaconstantinou

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Eucaryotic organisms possess natural defense processes triggered by stress factors such as injury, infection, and inflammation. The acute phase response is an early defense mechanism during which striking changes in protein synthesis occur in the liver and other tissues. The altered expression of many acute phase protein genes is at the transcriptional level. Some of these genes have DNA binding sites for the CCAAT/enhancer binding protein (C/EBP) family of transcription factors. We report here that in vivo expression of three isoforms of C/EBP is dramatically changed during the acute phase response. The steady-state mRNA levels of C/EBPα decreased significantly in the liver, lung, and fat tissues of lipopolysaccharide (LPS)-treated mice; moreover, nuclear run-off transcription assays indicated a decrease in the rate of C/ EBPa gene transcription in isolated liver nuclei. The steady-state levels of C/EBPβ and a new isoform, C/ EBPδ, were dramatically increased in many tissues within 4 h following LPS treatment. The rates of transcription of these two genes were only minimally altered in liver but significantly increased in kidney nuclei isolated from stimulated animals. Thus, the C/ EBP isoforms exhibit differential mechanisms in their responses to LPS in various tissues and are likely to play an important role in mediating the transcriptional activation of genes involved in the acute phase response.

Original languageEnglish (US)
Pages (from-to)5021-5024
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number8
StatePublished - Mar 15 1992

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CCAAT-Enhancer-Binding Proteins
Acute-Phase Reaction
Protein Isoforms
Genes
Liver
Tissue
Transcription
Lipopolysaccharides
Acute-Phase Proteins
Transcriptional Activation
Assays
Animals
Transcription Factors
Chemical activation
Fats
Binding Sites
Inflammation
Kidney
Lung
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential expression of three C/EBP isoforms in multiple tissues during the acute phase response. / Alam, Tawfiq; An, Mi Ra; Papaconstantinou, John.

In: Journal of Biological Chemistry, Vol. 267, No. 8, 15.03.1992, p. 5021-5024.

Research output: Contribution to journalArticle

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