Differential histone modification and protein expression associated with cell wall removal and regeneration in rice (Oryza sativa)

Feng Tan, Kangling Zhang, Hana Mujahid, Desh Pal S Verma, Zhaohua Peng

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The cell wall is a critical extracellular structure that provides protection and structural support in plant cells. To study the biological function of the cell wall and the regulation of cell wall resynthesis, we examined cellular responses to enzymatic removal of the cell wall in rice (Oryza sativa) suspension cells using proteomic approaches. We find that removal of cell wall stimulates cell wall synthesis from multiple sites in protoplasts instead of from a single site as in cytokinesis. Nucleus DAPI stain and MNase digestion further show that removal of the cell wall is concomitant with substantial chromatin reorganization. Histone post-translational modification studies using both Western blots and isotope labeling assisted quantitative mass spectrometry analyses reveal that substantial histone modification changes, particularly H3K18 AC and H3K23 AC, are associated with the removal and regeneration of the cell wall. Label-free quantitative proteome analyses further reveal that chromatin associated proteins undergo dramatic changes upon removal of the cell wall, along with cytoskeleton, cell wall metabolism, and stress-response proteins. This study demonstrates that cell wall removal is associated with substantial chromatin change and may lead to stimulation of cell wall synthesis using a novel mechanism.

Original languageEnglish (US)
Pages (from-to)551-563
Number of pages13
JournalJournal of Proteome Research
Volume10
Issue number2
DOIs
StatePublished - Feb 4 2011
Externally publishedYes

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Histone Code
Histones
Cell Wall
Regeneration
Cells
Proteins
Chromatin
Oryza
Isotope Labeling
Cytokinesis
Protoplasts
Plant Cells
Proteome
Post Translational Protein Processing
Heat-Shock Proteins
Cytoskeleton
Metabolism
Isotopes
Proteomics

Keywords

  • Cell wall regeneration
  • Chromatin reorganization
  • Comparative proteomics
  • Gene annotation
  • Histone post-translational modification
  • Protoplast
  • Quantitative histone H3 acetylation
  • Shotgun proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Differential histone modification and protein expression associated with cell wall removal and regeneration in rice (Oryza sativa). / Tan, Feng; Zhang, Kangling; Mujahid, Hana; Verma, Desh Pal S; Peng, Zhaohua.

In: Journal of Proteome Research, Vol. 10, No. 2, 04.02.2011, p. 551-563.

Research output: Contribution to journalArticle

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