Differential incorporation of 5-bromodeoxyuridine into DNA puffs of larval salivary gland chromosomes in Rhynchosciara

William S. Bradshaw, John Papaconstantinou

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An autoradiographic analysis of the incorporation of 5-bromo-2′-deoxyuridine (BUdR) into salivary gland chromosomes of Rhynchosciara larvae has demonstrated differential synthesis of DNA in a specific set of chromosomal puffs. DNA isolated from larval salivary glands at the specific stage in development when these DNA puffs occur has also been subjected to isopycnic centrifugation in CsCl. The profiles of these gradients are heterodisperse and exhibit regions of very high density shifted away from the bulk DNA. The technique makes possible the isolation and purification of sequences unique to a specific set of chromosomal loci.

Original languageEnglish (US)
Pages (from-to)306-312
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume41
Issue number2
DOIs
StatePublished - Oct 23 1970
Externally publishedYes

Fingerprint

Bromodeoxyuridine
Chromosomes
Salivary Glands
DNA
Chromosomal Puffs
Isopycnic Centrifugation
Centrifugation
Purification
Larva

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - An autoradiographic analysis of the incorporation of 5-bromo-2′-deoxyuridine (BUdR) into salivary gland chromosomes of Rhynchosciara larvae has demonstrated differential synthesis of DNA in a specific set of chromosomal puffs. DNA isolated from larval salivary glands at the specific stage in development when these DNA puffs occur has also been subjected to isopycnic centrifugation in CsCl. The profiles of these gradients are heterodisperse and exhibit regions of very high density shifted away from the bulk DNA. The technique makes possible the isolation and purification of sequences unique to a specific set of chromosomal loci.

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