An autoradiographic analysis of the incorporation of 5-bromo-2′-deoxyuridine (BUdR) into salivary gland chromosomes of Rhynchosciara larvae has demonstrated differential synthesis of DNA in a specific set of chromosomal puffs. DNA isolated from larval salivary glands at the specific stage in development when these DNA puffs occur has also been subjected to isopycnic centrifugation in CsCl. The profiles of these gradients are heterodisperse and exhibit regions of very high density shifted away from the bulk DNA. The technique makes possible the isolation and purification of sequences unique to a specific set of chromosomal loci.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 23 1970|
ASJC Scopus subject areas
- Molecular Biology