TY - JOUR
T1 - Differential incorporation of 5-bromodeoxyuridine into DNA puffs of larval salivary gland chromosomes in Rhynchosciara
AU - Bradshaw, William S.
AU - Papaconstantinou, John
N1 - Funding Information:
1 Research sponsored by the U. Union Carbide Corporation.
PY - 1970/10/23
Y1 - 1970/10/23
N2 - An autoradiographic analysis of the incorporation of 5-bromo-2′-deoxyuridine (BUdR) into salivary gland chromosomes of Rhynchosciara larvae has demonstrated differential synthesis of DNA in a specific set of chromosomal puffs. DNA isolated from larval salivary glands at the specific stage in development when these DNA puffs occur has also been subjected to isopycnic centrifugation in CsCl. The profiles of these gradients are heterodisperse and exhibit regions of very high density shifted away from the bulk DNA. The technique makes possible the isolation and purification of sequences unique to a specific set of chromosomal loci.
AB - An autoradiographic analysis of the incorporation of 5-bromo-2′-deoxyuridine (BUdR) into salivary gland chromosomes of Rhynchosciara larvae has demonstrated differential synthesis of DNA in a specific set of chromosomal puffs. DNA isolated from larval salivary glands at the specific stage in development when these DNA puffs occur has also been subjected to isopycnic centrifugation in CsCl. The profiles of these gradients are heterodisperse and exhibit regions of very high density shifted away from the bulk DNA. The technique makes possible the isolation and purification of sequences unique to a specific set of chromosomal loci.
UR - http://www.scopus.com/inward/record.url?scp=0014959902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014959902&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(70)90504-8
DO - 10.1016/0006-291X(70)90504-8
M3 - Article
C2 - 4931929
AN - SCOPUS:0014959902
SN - 0006-291X
VL - 41
SP - 306
EP - 312
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -