Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal

I. Khan, Amjad Hossain, G. F. Whitman, P. G. McDonough, N. H. Sarkar

Research output: Contribution to journalArticle

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Abstract

The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalProstaglandins
Volume46
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Dinoprost
Uterus
Rats
Ovary
Rat control
jun Genes
Tissue
Luteolysis
Corpus Luteum
Liquid nitrogen
Subcutaneous Injections
Gene expression
Northern Blotting
Animals
Nitrogen
Steroids
RNA
Gene Expression
Injections

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal. / Khan, I.; Hossain, Amjad; Whitman, G. F.; McDonough, P. G.; Sarkar, N. H.

In: Prostaglandins, Vol. 46, No. 2, 1993, p. 139-144.

Research output: Contribution to journalArticle

Khan, I. ; Hossain, Amjad ; Whitman, G. F. ; McDonough, P. G. ; Sarkar, N. H. / Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal. In: Prostaglandins. 1993 ; Vol. 46, No. 2. pp. 139-144.
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