Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal

I. Khan; A. Hossain; G. F. Whitman; P. G. McDonough; N. H. Sarkar

doi:10.1016/0090-6980(93)90039-A

Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal

I. Khan
, A. Hossain
, G. F. Whitman
, P. G. McDonough
, N. H. Sarkar

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.

Original language	English (US)
Pages (from-to)	139-144
Number of pages	6
Journal	Prostaglandins
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/0090-6980(93)90039-A
State	Published - Aug 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology

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@article{4ea38542d31440c795009053dd719a56,

title = "Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal",

abstract = "The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.",

author = "I. Khan and A. Hossain and Whitman, \{G. F.\} and McDonough, \{P. G.\} and Sarkar, \{N. H.\}",

note = "Funding Information: This work was supported by a research grant to lqbal Khan, Ph.D. from the Department of Obstetrics and Gynecology, Medical College of Georgia.",

year = "1993",

month = aug,

doi = "10.1016/0090-6980(93)90039-A",

language = "English (US)",

volume = "46",

pages = "139--144",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal

AU - Khan, I.

AU - Hossain, A.

AU - Whitman, G. F.

AU - McDonough, P. G.

AU - Sarkar, N. H.

N1 - Funding Information: This work was supported by a research grant to lqbal Khan, Ph.D. from the Department of Obstetrics and Gynecology, Medical College of Georgia.

PY - 1993/8

Y1 - 1993/8

N2 - The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.

AB - The influence of PGF2∝ on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 μg PGF2∝ by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrified 30 minutes after the second injection of PGF2∝. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2∝ treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2∝ on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2∝ in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2∝ in luteolysis.

UR - https://www.scopus.com/pages/publications/0027257101

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U2 - 10.1016/0090-6980(93)90039-A

DO - 10.1016/0090-6980(93)90039-A

M3 - Article

C2 - 8210443

AN - SCOPUS:0027257101

SN - 0090-6980

VL - 46

SP - 139

EP - 144

JO - Prostaglandins

JF - Prostaglandins

IS - 2

ER -

Differential induction of c-jun expression by PGF2-alpha in rat ovary, uterus and adrenal

Abstract

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