Differential induction of polyamine oxidase activity in liver and heart of iron-overloaded rats

U. R. Tipnis, G. Y. He, M Khan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present study was undertaken to investigate the effect of iron dextran treatment on polyamine oxidase (PAO) activity iron accumulation, and lipid peroxidation in livers and hearts of rats. PAO catalyzes oxidative deamination of polyamines, the cellular aliphatic cations. This reaction produces highly toxic hydrogen peroxide, 3-acetamidopropanal, and precursors of higher polyamines. The rats were given iron dextran daily for 7 d. In iron-dextran-treated rats, a marked increase in the hepatic level of iron was associated with enhanced lipid peroxidation and increased PAO activity. Though iron accumulation and lipid peroxidation in the iron-treated rats increased significantly in the heart, PAO activity remained unchanged. The paraffin sections of livers stained with Perls iron stain showed the presence of iron in macrophages and hepatocytes. The sections of hearts showed iron deposits only in macrophages, while myocytes showed no iron staining. These results show that although iron dextran treatment results in accumulation of iron in both liver and heart, it induces PAO activity only in liver. The significance of increased PAO activity in lipid peroxidation and fibrosis in iron-mediated injury is discussed.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalJournal of Toxicology and Environmental Health
Volume51
Issue number3
DOIs
StatePublished - 1997

Fingerprint

Liver
Rats
Iron
iron
Dextran
Dextrans
Lipids
Lipid Peroxidation
lipid
Macrophages
Polyamines
polyamine oxidase
Oxidoreductases
Iron deposits
Deamination
Poisons
Hydrogen peroxide
Paraffin
Paraffins
Hydrogen Peroxide

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Differential induction of polyamine oxidase activity in liver and heart of iron-overloaded rats. / Tipnis, U. R.; He, G. Y.; Khan, M.

In: Journal of Toxicology and Environmental Health, Vol. 51, No. 3, 1997, p. 235-244.

Research output: Contribution to journalArticle

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