Differential microbicidal effects of human histone proteins H2A and H2B on Leishmania promastigotes and amastigotes

Yingwei Wang, Yang Chen, Lijun Xin, Stephen M. Beverley, Eric D. Carlsen, Vsevolod Popov, Kwang Poo Chang, Ming Wang, Lynn Soong

Research output: Contribution to journalArticle

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Abstract

Recent studies have shown that histone proteins can act as antimicrobial peptides in host defense against extracellular bacteria, fungi, and Leishmania promastigotes. In this study, we used human recombinant histone proteins to further study their leishmaniacidal effects and the underlying mechanisms. We found that the histones H2A and H2B (but not H10) could directly and efficiently kill promastigotes of Leishmania amazonensis, L. major, L. braziliensis, and L. mexicana in a treatment dose-dependent manner. Scanning electron microscopy revealed surface disruption of histone-treated promastigotes. More importantly, the preexposure of promastigotes to histone proteins markedly decreased the infectivity of promastigotes to murine macrophages (Mφs) in vitro. However, axenic and lesion-derived amastigotes of L. amazonensis and L. mexicana were relatively resistant to histone treatment, which correlated with the low levels of intracellular H2A in treated amastigotes. To understand the mechanisms underlying these differential responses, we investigated the role of promastigote surface molecules in histone-mediated killing. Compared with the corresponding controls, transgenic L. amazonensis promastigotes expressing lower levels of surface gp63 proteins were more susceptible to histone H2A, while L. major and L. mexicana promastigotes with targeted deletion of the lipophosphoglycan 2 (lpg2) gene (but not the lpg1 gene) were more resistant to histone H2A. We discuss the influence of promastigote major surface molecules in the leishmaniacidal effect of histone proteins. This study provides new information on host innate immunity to different developmental stages of Leishmania parasites.

Original languageEnglish (US)
Pages (from-to)1124-1133
Number of pages10
JournalInfection and Immunity
Volume79
Issue number3
DOIs
StatePublished - Mar 2011

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Leishmania
Histones
Proteins
Recombinant Proteins
Innate Immunity
Electron Scanning Microscopy
Genes
Membrane Proteins
Parasites
Fungi
Macrophages
Bacteria
Peptides

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

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Differential microbicidal effects of human histone proteins H2A and H2B on Leishmania promastigotes and amastigotes. / Wang, Yingwei; Chen, Yang; Xin, Lijun; Beverley, Stephen M.; Carlsen, Eric D.; Popov, Vsevolod; Chang, Kwang Poo; Wang, Ming; Soong, Lynn.

In: Infection and Immunity, Vol. 79, No. 3, 03.2011, p. 1124-1133.

Research output: Contribution to journalArticle

Wang, Yingwei ; Chen, Yang ; Xin, Lijun ; Beverley, Stephen M. ; Carlsen, Eric D. ; Popov, Vsevolod ; Chang, Kwang Poo ; Wang, Ming ; Soong, Lynn. / Differential microbicidal effects of human histone proteins H2A and H2B on Leishmania promastigotes and amastigotes. In: Infection and Immunity. 2011 ; Vol. 79, No. 3. pp. 1124-1133.
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