Differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers

Effect of human cytomegalovirus infection

Thomas Albrecht, Cheng Zong Deng, Sherif Abdel-Rahman, Michael Fons, Paul Cinciripini, Randa A. El-Zein

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We used the mutagen sensitivity assay to test the hypothesis that human cytomegalovirus (HCMV) infection modifies the sensitivity of cells to genetic damage from genotoxic agents. Chromosome aberration (CA) frequency in peripheral blood lymphocytes (PBLs) from 20 smokers who were matched with 20 nonsmokers by age (± 5 years), sex, and ethnicity was evaluated following in vitro exposure to bleomycin and/or HCMV infection. Bleomycin induced significant (P < 0.05) concentration-dependent increases in the frequency of aberrant cells, chromatid-type damage (breaks), and chromosome-type aberrations (deletions, rearrangements) in PBLs. The baseline (background) CA frequency was similar in both smokers and nonsmokers. Significantly higher frequencies of aberrant cells (P < 0.05) were observed in PBLs from smokers compared to nonsmokers at all bleomycin concentrations tested (10, 30 and 100 μg/ml). Infection of PBLs with HCMV induced a significant (P < 0.05) twofold increase in the frequency of CA (primarily chromatid breaks) in PBLs, regardless of the smoking status. PBLs from smokers and nonsmokers infected with HCMV prior to challenge with bleomycin demonstrated significant (P < 0.05) concentration-dependent increases in the levels of aberrant cells, chromatid-type damage (breaks), and chromosome-type aberrations (deletions, rearrangements) compared to noninfected cells challenged with bleomycin. The frequency of induced CA was consistently higher for PBLs derived from smokers relative to nonsmokers (P = 0.06 and 0.002). These data indicate that, individually, both smoking and HCMV infection significantly enhance the sensitivity of PBLs to bleomycin-induced genetic damage. More importantly, the date also suggest that smoking and HCMV infection interact synergistically to enhance the sensitivity of PBLs to such damage. Environ. Mol. Mutagen.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalEnvironmental and Molecular Mutagenesis
Volume43
Issue number3
DOIs
StatePublished - 2004

Fingerprint

Lymphocytes
Mutagens
Cytomegalovirus Infections
Blood
blood
Bleomycin
Chromosomes
Aberrations
Chromosome Aberrations
chromosome
Chromatids
damage
smoking
Chromosome Deletion
Smoking
Cytomegalovirus
infection
mutagen
effect
ethnicity

Keywords

  • Bleomycin
  • Chromosome aberrations
  • Cytomegalovirus
  • Mutagen sensitivity
  • Smoking

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers : Effect of human cytomegalovirus infection. / Albrecht, Thomas; Deng, Cheng Zong; Abdel-Rahman, Sherif; Fons, Michael; Cinciripini, Paul; El-Zein, Randa A.

In: Environmental and Molecular Mutagenesis, Vol. 43, No. 3, 2004, p. 169-178.

Research output: Contribution to journalArticle

Albrecht, Thomas ; Deng, Cheng Zong ; Abdel-Rahman, Sherif ; Fons, Michael ; Cinciripini, Paul ; El-Zein, Randa A. / Differential mutagen sensitivity of peripheral blood lymphocytes from smokers and nonsmokers : Effect of human cytomegalovirus infection. In: Environmental and Molecular Mutagenesis. 2004 ; Vol. 43, No. 3. pp. 169-178.
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