Differential Regulation of Human T Lymphocyte Protein Kinase C Activity by Unsaturated Fatty Acids

Ronald G. Rossetti, Christina M. Seiler, Michael Laposata, Robert B. Zurier

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Administration of γ-linolenic acid suppresses active synovitis in patients with rheumatoid arthritis. We therefore examined the effects of γ-linolenic acid and its first metabolite, dihomo-γ-linolenic acid, on protein kinase C, a key element in transduction of signals from cell surface to nucleus. We report here that γ-linolenic acid and dihomo-γ-linolenic acid suppress total protein kinase C activity, but facilitate translocation of protein kinase C activity from cytosol to membrane in human peripheral blood T lymphocytes stimulated with phorbol 12-myristate 13-acetate. Arachidonic acid and eicosapentaenoic acid do not influence total protein kinase C activity and have only modest effects on enzyme translocation. These findings in whole cells are in contrast to results of experiments performed with isolated protein kinase C, in which unsaturated fatty acids uniformly enhance protein kinase C activity. The differential effects of unsaturated fatty acids underscore the complexity of protein kinase C regulation and indicate that γ-linolenic and dihomo-γ-linolenic acids influence T lymphocyte protein kinase C metabolism in a manner that is unique among unsaturated fatty acid precursors of eicosanoids.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalClinical Immunology and Immunopathology
Volume76
Issue number3
DOIs
StatePublished - Sep 1995
Externally publishedYes

Fingerprint

Unsaturated Fatty Acids
Protein Kinase C
alpha-Linolenic Acid
T-Lymphocytes
Linolenic Acids
Eicosapentaenoic Acid
Synovitis
Eicosanoids
Brachyury protein
Arachidonic Acid
Cytosol
Signal Transduction
Rheumatoid Arthritis
Acetates
Membranes
Enzymes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Immunology

Cite this

Differential Regulation of Human T Lymphocyte Protein Kinase C Activity by Unsaturated Fatty Acids. / Rossetti, Ronald G.; Seiler, Christina M.; Laposata, Michael; Zurier, Robert B.

In: Clinical Immunology and Immunopathology, Vol. 76, No. 3, 09.1995, p. 220-224.

Research output: Contribution to journalArticle

Rossetti, Ronald G. ; Seiler, Christina M. ; Laposata, Michael ; Zurier, Robert B. / Differential Regulation of Human T Lymphocyte Protein Kinase C Activity by Unsaturated Fatty Acids. In: Clinical Immunology and Immunopathology. 1995 ; Vol. 76, No. 3. pp. 220-224.
@article{9097530d55c74f3bb52eeec9518f3098,
title = "Differential Regulation of Human T Lymphocyte Protein Kinase C Activity by Unsaturated Fatty Acids",
abstract = "Administration of γ-linolenic acid suppresses active synovitis in patients with rheumatoid arthritis. We therefore examined the effects of γ-linolenic acid and its first metabolite, dihomo-γ-linolenic acid, on protein kinase C, a key element in transduction of signals from cell surface to nucleus. We report here that γ-linolenic acid and dihomo-γ-linolenic acid suppress total protein kinase C activity, but facilitate translocation of protein kinase C activity from cytosol to membrane in human peripheral blood T lymphocytes stimulated with phorbol 12-myristate 13-acetate. Arachidonic acid and eicosapentaenoic acid do not influence total protein kinase C activity and have only modest effects on enzyme translocation. These findings in whole cells are in contrast to results of experiments performed with isolated protein kinase C, in which unsaturated fatty acids uniformly enhance protein kinase C activity. The differential effects of unsaturated fatty acids underscore the complexity of protein kinase C regulation and indicate that γ-linolenic and dihomo-γ-linolenic acids influence T lymphocyte protein kinase C metabolism in a manner that is unique among unsaturated fatty acid precursors of eicosanoids.",
author = "Rossetti, {Ronald G.} and Seiler, {Christina M.} and Michael Laposata and Zurier, {Robert B.}",
year = "1995",
month = "9",
doi = "10.1006/clin.1995.1119",
language = "English (US)",
volume = "76",
pages = "220--224",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Differential Regulation of Human T Lymphocyte Protein Kinase C Activity by Unsaturated Fatty Acids

AU - Rossetti, Ronald G.

AU - Seiler, Christina M.

AU - Laposata, Michael

AU - Zurier, Robert B.

PY - 1995/9

Y1 - 1995/9

N2 - Administration of γ-linolenic acid suppresses active synovitis in patients with rheumatoid arthritis. We therefore examined the effects of γ-linolenic acid and its first metabolite, dihomo-γ-linolenic acid, on protein kinase C, a key element in transduction of signals from cell surface to nucleus. We report here that γ-linolenic acid and dihomo-γ-linolenic acid suppress total protein kinase C activity, but facilitate translocation of protein kinase C activity from cytosol to membrane in human peripheral blood T lymphocytes stimulated with phorbol 12-myristate 13-acetate. Arachidonic acid and eicosapentaenoic acid do not influence total protein kinase C activity and have only modest effects on enzyme translocation. These findings in whole cells are in contrast to results of experiments performed with isolated protein kinase C, in which unsaturated fatty acids uniformly enhance protein kinase C activity. The differential effects of unsaturated fatty acids underscore the complexity of protein kinase C regulation and indicate that γ-linolenic and dihomo-γ-linolenic acids influence T lymphocyte protein kinase C metabolism in a manner that is unique among unsaturated fatty acid precursors of eicosanoids.

AB - Administration of γ-linolenic acid suppresses active synovitis in patients with rheumatoid arthritis. We therefore examined the effects of γ-linolenic acid and its first metabolite, dihomo-γ-linolenic acid, on protein kinase C, a key element in transduction of signals from cell surface to nucleus. We report here that γ-linolenic acid and dihomo-γ-linolenic acid suppress total protein kinase C activity, but facilitate translocation of protein kinase C activity from cytosol to membrane in human peripheral blood T lymphocytes stimulated with phorbol 12-myristate 13-acetate. Arachidonic acid and eicosapentaenoic acid do not influence total protein kinase C activity and have only modest effects on enzyme translocation. These findings in whole cells are in contrast to results of experiments performed with isolated protein kinase C, in which unsaturated fatty acids uniformly enhance protein kinase C activity. The differential effects of unsaturated fatty acids underscore the complexity of protein kinase C regulation and indicate that γ-linolenic and dihomo-γ-linolenic acids influence T lymphocyte protein kinase C metabolism in a manner that is unique among unsaturated fatty acid precursors of eicosanoids.

UR - http://www.scopus.com/inward/record.url?scp=0029025996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029025996&partnerID=8YFLogxK

U2 - 10.1006/clin.1995.1119

DO - 10.1006/clin.1995.1119

M3 - Article

VL - 76

SP - 220

EP - 224

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 3

ER -