Differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus

Antonieta Guerrero-Plata, Antonella Casola, Giovanni Suarez, Xiang Yu, LeAnne Spetch, Mark E. Peeples, Roberto Garofalo

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Dendritic cells (DCs) play a pivotal role in shaping antiviral immune responses in the respiratory tract. Human metapneumovirus (hMPV) is a recently identified pathogen and like its better known relative, respiratory syncytial virus (RSV), has been increasingly recognized as a major cause of respiratory morbidity in infants and in elderly persons. In the present study, we examined susceptibility as well as cellular responses of human DCs to hMPV compared with RSV. Monocyte-derived DCs (moDCs) were susceptible to infection by both viruses, but only RSV was able to induce a productive infection with release of viral progeny. Despite the fact that viral infection resulted in phenotypic maturation of moDCs, as shown by the upregulation of cell surface markers and antigen-presenting molecules (MHC I and II, CD80, CD83, CD86, CD38), RSV-infected moDCs showed a severely impaired capacity to stimulate CD4 + T cell proliferation. Compared with hMPV, RSV was a more potent inducer of inflammatory and immunomodulatory cytokines, including TNF-α, IL-6, IL-1β, IL-10, and IL-12p70 in both moDCs and plasmacytoid dendritic cells (pDCs). On the other hand, hMPV, but not RSV, was able to trigger production of IFN-α by moDCs, while both viruses strongly induced IFN-α in pDCs. Finally, both viruses strikingly suppressed IFN-α production by moDCs or pDCs stimulated with synthetic dsRNA and CpG-ODN, respectively. The findings provide novel evidence that RSV and hMPV differentially activate human DCs and may use distinct mechanisms to interfere with the host innate and adaptive immune responses.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume34
Issue number3
DOIs
StatePublished - Mar 2006

Fingerprint

Human respiratory syncytial virus
Metapneumovirus
Respiratory Syncytial Viruses
Viruses
Dendritic Cells
Monocytes
Virus Diseases
Differentiation Antigens
Adaptive Immunity
Surface Antigens
Interleukin-1
Innate Immunity
Respiratory System
Interleukin-10
Antiviral Agents
Interleukin-6
T-cells
Up-Regulation
Cell proliferation
Pathogens

Keywords

  • Dendritic cells
  • Human metapneumovirus
  • Innate immunity
  • Interferon type I
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus. / Guerrero-Plata, Antonieta; Casola, Antonella; Suarez, Giovanni; Yu, Xiang; Spetch, LeAnne; Peeples, Mark E.; Garofalo, Roberto.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 34, No. 3, 03.2006, p. 320-329.

Research output: Contribution to journalArticle

Guerrero-Plata, Antonieta ; Casola, Antonella ; Suarez, Giovanni ; Yu, Xiang ; Spetch, LeAnne ; Peeples, Mark E. ; Garofalo, Roberto. / Differential response of dendritic cells to human metapneumovirus and respiratory syncytial virus. In: American Journal of Respiratory Cell and Molecular Biology. 2006 ; Vol. 34, No. 3. pp. 320-329.
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