Differential response to gamma radiation of human stomach cancer cells in vitro

V. K. Jenkins, S. C. Barranco, Courtney Townsend, R. R. Perry, K. L. Ives

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In vitro effects of radiation were studied in two permanent cell lines (AGS and SII) from two patients with adenocarcinoma of the stomach and three permanent sublines from each cell line. Radiation survival parameters for AGS and SII parent cell lines and sublines were determined after in vitro irradiation of their cells with 0·5 to 10 Gy of 60Co gamma rays. The AGS and SII cell lines had different growth properties, DNA contents and radiation survival curves. Surviving fractions of SII parent cells (76 chromosomes) after 2·0 and 10 Gy were 1·22 and 17·8 times greater, respectively, than values for AGS parent cells (47 chromosomes). Sensitivities (D0) were 1·08 and 1·45 Gy for AGS and SII parent lines, respectively. The D0 values for AGS parent cells and sublines were similar (1·01 to 1·08 Gy), but SII parent cells and sublines had D0 values of 1·45, 1·36, 1·37 and 1·12 Gy (for SII-A). Also, the SII parent cells had survival fractions after 2·0 and 10 Gy that were 1·3 and 11·3 times greater, respectively, than values for the SII-A cells. These data show differences in radiation responses among stomach cancer cell lines and sublines that may relate to DNA content, but there was no consistent correlation between radiation response and a particular cell characteristic.

Original languageEnglish (US)
Pages (from-to)269-278
Number of pages10
JournalInternational Journal of Radiation Biology
Volume50
Issue number2
DOIs
StatePublished - 1986

Fingerprint

stomach
stomach neoplasms
Gamma Rays
Gamma rays
Stomach Neoplasms
gamma radiation
cancer
Cells
gamma rays
cultured cells
Radiation
cell lines
cells
Cell Line
Chromosomes
radiation
chromosomes
DNA
Chromosomes, Human, Pair 20
deoxyribonucleic acid

Keywords

  • Stomach cancer cells
  • Survival

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Differential response to gamma radiation of human stomach cancer cells in vitro. / Jenkins, V. K.; Barranco, S. C.; Townsend, Courtney; Perry, R. R.; Ives, K. L.

In: International Journal of Radiation Biology, Vol. 50, No. 2, 1986, p. 269-278.

Research output: Contribution to journalArticle

Jenkins, V. K. ; Barranco, S. C. ; Townsend, Courtney ; Perry, R. R. ; Ives, K. L. / Differential response to gamma radiation of human stomach cancer cells in vitro. In: International Journal of Radiation Biology. 1986 ; Vol. 50, No. 2. pp. 269-278.
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