Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(L/DL-lactide) membranes

Zbigniew Gugala, Sylwester Gogolewski

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Nonporous and porous membranes produced from poly(L/DL-lactide) 80/20% were characterized using profilometry, contact-angle measurements, infra-red spectroscopy, X-ray photoemission spectroscopy and scanning electron microscopy, and used to culture bone marrow stromal cells isolated from the rat femora. The cells were cultured for 5, 10, 15 and 20 days. Cell growth and activity was estimated from the amounts of DNA, alkaline phosphatase activity and total protein amount present in the cell lysate and cell differentiation was assessed histochemically. Cell morphology was estimated from scanning electron microscopy. The cells fully expressed osteoblastic phenotype, revealed spindle-shaped, ellipsoidal morphology, developed podia, produced an abundant fibrillar extracellular matrix and mineral noduli. The number of cells on the membranes increased with time of culturing and was higher for the porous membranes than the nonporous membranes. Osteoblastic differentiation was most significant between 5 and 10 days of culture. The total amounts of DNA, alkaline phosphatase and proteins increased with time of culturing. The surface characteristics of the porous membranes were superior to the nonporous membranes.

Original languageEnglish (US)
Pages (from-to)2299-2307
Number of pages9
JournalBiomaterials
Volume25
Issue number12
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Rats
Bone
Membranes
Growth
Electron Scanning Microscopy
Phosphatases
Alkaline Phosphatase
DNA
Photoelectron Spectroscopy
Proteins
Scanning electron microscopy
Profilometry
Femur
Cell growth
Extracellular Matrix
Minerals
Angle measurement
Photoelectron spectroscopy
Cell Differentiation

Keywords

  • Bone marrow stromal cells
  • Cell culture
  • Osteoblast differentiation
  • Polylactide membrane
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(L/DL-lactide) membranes. / Gugala, Zbigniew; Gogolewski, Sylwester.

In: Biomaterials, Vol. 25, No. 12, 05.2004, p. 2299-2307.

Research output: Contribution to journalArticle

@article{22e8e4184371435a8b2cd019cbc9fe04,
title = "Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(L/DL-lactide) membranes",
abstract = "Nonporous and porous membranes produced from poly(L/DL-lactide) 80/20{\%} were characterized using profilometry, contact-angle measurements, infra-red spectroscopy, X-ray photoemission spectroscopy and scanning electron microscopy, and used to culture bone marrow stromal cells isolated from the rat femora. The cells were cultured for 5, 10, 15 and 20 days. Cell growth and activity was estimated from the amounts of DNA, alkaline phosphatase activity and total protein amount present in the cell lysate and cell differentiation was assessed histochemically. Cell morphology was estimated from scanning electron microscopy. The cells fully expressed osteoblastic phenotype, revealed spindle-shaped, ellipsoidal morphology, developed podia, produced an abundant fibrillar extracellular matrix and mineral noduli. The number of cells on the membranes increased with time of culturing and was higher for the porous membranes than the nonporous membranes. Osteoblastic differentiation was most significant between 5 and 10 days of culture. The total amounts of DNA, alkaline phosphatase and proteins increased with time of culturing. The surface characteristics of the porous membranes were superior to the nonporous membranes.",
keywords = "Bone marrow stromal cells, Cell culture, Osteoblast differentiation, Polylactide membrane, Tissue engineering",
author = "Zbigniew Gugala and Sylwester Gogolewski",
year = "2004",
month = "5",
doi = "10.1016/j.biomaterials.2003.09.009",
language = "English (US)",
volume = "25",
pages = "2299--2307",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "12",

}

TY - JOUR

T1 - Differentiation, growth and activity of rat bone marrow stromal cells on resorbable poly(L/DL-lactide) membranes

AU - Gugala, Zbigniew

AU - Gogolewski, Sylwester

PY - 2004/5

Y1 - 2004/5

N2 - Nonporous and porous membranes produced from poly(L/DL-lactide) 80/20% were characterized using profilometry, contact-angle measurements, infra-red spectroscopy, X-ray photoemission spectroscopy and scanning electron microscopy, and used to culture bone marrow stromal cells isolated from the rat femora. The cells were cultured for 5, 10, 15 and 20 days. Cell growth and activity was estimated from the amounts of DNA, alkaline phosphatase activity and total protein amount present in the cell lysate and cell differentiation was assessed histochemically. Cell morphology was estimated from scanning electron microscopy. The cells fully expressed osteoblastic phenotype, revealed spindle-shaped, ellipsoidal morphology, developed podia, produced an abundant fibrillar extracellular matrix and mineral noduli. The number of cells on the membranes increased with time of culturing and was higher for the porous membranes than the nonporous membranes. Osteoblastic differentiation was most significant between 5 and 10 days of culture. The total amounts of DNA, alkaline phosphatase and proteins increased with time of culturing. The surface characteristics of the porous membranes were superior to the nonporous membranes.

AB - Nonporous and porous membranes produced from poly(L/DL-lactide) 80/20% were characterized using profilometry, contact-angle measurements, infra-red spectroscopy, X-ray photoemission spectroscopy and scanning electron microscopy, and used to culture bone marrow stromal cells isolated from the rat femora. The cells were cultured for 5, 10, 15 and 20 days. Cell growth and activity was estimated from the amounts of DNA, alkaline phosphatase activity and total protein amount present in the cell lysate and cell differentiation was assessed histochemically. Cell morphology was estimated from scanning electron microscopy. The cells fully expressed osteoblastic phenotype, revealed spindle-shaped, ellipsoidal morphology, developed podia, produced an abundant fibrillar extracellular matrix and mineral noduli. The number of cells on the membranes increased with time of culturing and was higher for the porous membranes than the nonporous membranes. Osteoblastic differentiation was most significant between 5 and 10 days of culture. The total amounts of DNA, alkaline phosphatase and proteins increased with time of culturing. The surface characteristics of the porous membranes were superior to the nonporous membranes.

KW - Bone marrow stromal cells

KW - Cell culture

KW - Osteoblast differentiation

KW - Polylactide membrane

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=0346499673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346499673&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2003.09.009

DO - 10.1016/j.biomaterials.2003.09.009

M3 - Article

C2 - 14741595

AN - SCOPUS:0346499673

VL - 25

SP - 2299

EP - 2307

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 12

ER -