Differentiation of filoviruses by electron microscopy

Thomas Geisbert, P. B. Jahrling

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.

Original languageEnglish (US)
Pages (from-to)129-150
Number of pages22
JournalVirus Research
Volume39
Issue number2-3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Democratic Republic of the Congo
Sudan
Electron Microscopy
Morphogenesis
Virion
Filoviridae
Ebolavirus
Nucleoproteins
Immunoelectron Microscopy
Endocytosis
Cell Membrane
Infection
Reston
Proteins

Keywords

  • Ebola
  • Electron microscopy
  • Filoviridae
  • Marburg
  • Reston
  • Ultrastructure

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases
  • Molecular Biology

Cite this

Differentiation of filoviruses by electron microscopy. / Geisbert, Thomas; Jahrling, P. B.

In: Virus Research, Vol. 39, No. 2-3, 1995, p. 129-150.

Research output: Contribution to journalArticle

Geisbert, Thomas ; Jahrling, P. B. / Differentiation of filoviruses by electron microscopy. In: Virus Research. 1995 ; Vol. 39, No. 2-3. pp. 129-150.
@article{ea69a0d44e8d4b2eb677c9e523f86488,
title = "Differentiation of filoviruses by electron microscopy",
abstract = "Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.",
keywords = "Ebola, Electron microscopy, Filoviridae, Marburg, Reston, Ultrastructure",
author = "Thomas Geisbert and Jahrling, {P. B.}",
year = "1995",
doi = "10.1016/0168-1702(95)00080-1",
language = "English (US)",
volume = "39",
pages = "129--150",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Differentiation of filoviruses by electron microscopy

AU - Geisbert, Thomas

AU - Jahrling, P. B.

PY - 1995

Y1 - 1995

N2 - Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.

AB - Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MBG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.

KW - Ebola

KW - Electron microscopy

KW - Filoviridae

KW - Marburg

KW - Reston

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0029591306&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029591306&partnerID=8YFLogxK

U2 - 10.1016/0168-1702(95)00080-1

DO - 10.1016/0168-1702(95)00080-1

M3 - Article

VL - 39

SP - 129

EP - 150

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 2-3

ER -