Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry

Kangling Zhang, Peter M. Yau, Patrick R. Jones

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The identification of modification tri-methylation and acetylation sites of histone H3 was investigated by mass spectrometry. Histone H3 was purified by HPLC from the core histones isolated from chicken erythrocytes, digested by trypsin and then analyzed by MALDI-TOF and tandem spectrometry. High mass accuracy (5ppm) MALDI-TOF mass measurements were used to detect the mass difference (0.0360 Da, 40 ppm) between the substitution of Lys of an acetyl group (42.0106) versus three methyl groups (42.0470). The results show that H3 lysine 9 is mono-, di- and tri-methylated, and none of the MS evidence supports a putative acetylation of histone lysine 9 in chicken erythrocytes.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages315-316
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Acetylation
Methylation
Histones
Lysine
Mass spectrometry
Trypsin
Spectrometry
Substitution reactions

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Zhang, K., Yau, P. M., & Jones, P. R. (2002). Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 315-316)

Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry. / Zhang, Kangling; Yau, Peter M.; Jones, Patrick R.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 315-316.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, K, Yau, PM & Jones, PR 2002, Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 315-316, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Zhang K, Yau PM, Jones PR. Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 315-316
Zhang, Kangling ; Yau, Peter M. ; Jones, Patrick R. / Differentiation of tri-methylation from acetylation of histone H3 lysine 9 by mass spectrometry. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 315-316
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