Direct actions of angiopoietin-1 on human endothelium: Evidence for network stabilization, cell survival, and interaction with other angiogenic growth factors

Andreas Papapetropoulos, Guillermo García-Cardeña, Thomas J. Dengler, Peter C. Maisonpierre, George D. Yancopoulos, William C. Sessa

Research output: Contribution to journalArticle

350 Citations (Scopus)

Abstract

Angiopoietin-1 (Ang-1) is a recently described angiogenic protein that activates the endothelial Tie 2 receptor. Disruption of the Ang-1 gene shows that it has an indispensable role in blood vessel development, but it is not clear what specific effects, if any, Ang-1 has on endothelial cell (EC) phenotypes. Here, we show that Ang-1 dose-dependently stabilizes HUVEC network organization for up to 48 hours; this action of Ang-1 is dependent on Tie-2 receptor activation, because a soluble form of the Tie2-, but not the Tie1-receptor, completely blocks the effects of Ang-1. Moreover, we show that Ang-1 potentiates the actions of other angiogenic growth factors. Ang-1 markedly increases the survival of vascular networks (up to 96 hours) exposed to either vascular endothelial growth factor or endothelial cell growth supplement, a form of acidic fibroblast growth factor. In addition, Ang-1 prevents apoptotic death in HUVEC triggered by withdrawal of endothelial cell growth supplement. Collectively, these data are consistent with the idea that Ang-1 directly acts on human EC and interacts with other angiogenic molecules to stabilize vascular structures by promoting the survival of differentiated ECs.

Original languageEnglish (US)
Pages (from-to)213-223
Number of pages11
JournalLaboratory Investigation
Volume79
Issue number2
StatePublished - Feb 1 1999
Externally publishedYes

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Angiopoietin-1
Angiogenesis Inducing Agents
Cell Communication
Endothelium
Cell Survival
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Blood Vessels
Angiogenic Proteins
human ANGPT1 protein
Fibroblast Growth Factor 1
Growth
Vascular Endothelial Growth Factor A

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Direct actions of angiopoietin-1 on human endothelium : Evidence for network stabilization, cell survival, and interaction with other angiogenic growth factors. / Papapetropoulos, Andreas; García-Cardeña, Guillermo; Dengler, Thomas J.; Maisonpierre, Peter C.; Yancopoulos, George D.; Sessa, William C.

In: Laboratory Investigation, Vol. 79, No. 2, 01.02.1999, p. 213-223.

Research output: Contribution to journalArticle

Papapetropoulos, Andreas ; García-Cardeña, Guillermo ; Dengler, Thomas J. ; Maisonpierre, Peter C. ; Yancopoulos, George D. ; Sessa, William C. / Direct actions of angiopoietin-1 on human endothelium : Evidence for network stabilization, cell survival, and interaction with other angiogenic growth factors. In: Laboratory Investigation. 1999 ; Vol. 79, No. 2. pp. 213-223.
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