Direct catecholaminergic innervation of primate spinothalamic tract neurons

K. N. Westlund, S. M. Carlton, D. Zhang, W. D. Willis

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    Catecholaminergic axonal varicosities identified by immunocytochemical staining for dopamine-beta-hydroxylase were observed at the light microscopic level apposing the somata of retrogradely labeled spinothalamic tract neurons in the monkey spinal cord. Three retrogradely labeled and two intracellularly labeled spinothalamic neurons were serially sectioned and examined at selected intervals at the electron microscopic level. Electron microscopic study revealed that axonal boutons directly contacted the somata and/or dendrites of lamina I, IV, and V spinothalamic tract neurons. All of the profiles apposing one of the retrogradely labeled lamina I spinothalamic tract neurons were categorized from eight planes of section spaced at 1-μm intervals. Of the 305 profiles counted that were adjacent to this soma, 17 (5.6%) stained positively for dopamine-beta-hydroxylase. Of these 17 appositions, three were followed in serial sections to confirm that they had synaptic thickenings and alignment of vesicles along the membrane contacting the spinothalamic tract soma. Catecholaminergic boutons were observed apposing the somata and dendrites of intracellularly filled STT cells characterized as high threshold and wide dynamic range neurons. These observations clearly indicate a direct innervation of spinothalamic tract neurons by catecholaminergic neurons, providing anatomical data to support previous physiological findings demonstrating that catecholamines modulate nociceptive transmission.

    Original languageEnglish (US)
    Pages (from-to)178-186
    Number of pages9
    JournalJournal of Comparative Neurology
    Volume299
    Issue number2
    StatePublished - 1990

    Fingerprint

    Spinothalamic Tracts
    Primates
    Carisoprodol
    Neurons
    Dopamine beta-Hydroxylase
    Dendrites
    Electrons
    Catecholamines
    Haplorhini
    Spinal Cord
    Staining and Labeling
    Light
    Membranes

    Keywords

    • catecholamines
    • dopamine-beta-hydroxylase
    • electron microscopy
    • immunocytochemistry
    • norepinephrine
    • spinal cord

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Westlund, K. N., Carlton, S. M., Zhang, D., & Willis, W. D. (1990). Direct catecholaminergic innervation of primate spinothalamic tract neurons. Journal of Comparative Neurology, 299(2), 178-186.

    Direct catecholaminergic innervation of primate spinothalamic tract neurons. / Westlund, K. N.; Carlton, S. M.; Zhang, D.; Willis, W. D.

    In: Journal of Comparative Neurology, Vol. 299, No. 2, 1990, p. 178-186.

    Research output: Contribution to journalArticle

    Westlund, KN, Carlton, SM, Zhang, D & Willis, WD 1990, 'Direct catecholaminergic innervation of primate spinothalamic tract neurons', Journal of Comparative Neurology, vol. 299, no. 2, pp. 178-186.
    Westlund, K. N. ; Carlton, S. M. ; Zhang, D. ; Willis, W. D. / Direct catecholaminergic innervation of primate spinothalamic tract neurons. In: Journal of Comparative Neurology. 1990 ; Vol. 299, No. 2. pp. 178-186.
    @article{08ab12f3106c4379bb2765712eabc014,
    title = "Direct catecholaminergic innervation of primate spinothalamic tract neurons",
    abstract = "Catecholaminergic axonal varicosities identified by immunocytochemical staining for dopamine-beta-hydroxylase were observed at the light microscopic level apposing the somata of retrogradely labeled spinothalamic tract neurons in the monkey spinal cord. Three retrogradely labeled and two intracellularly labeled spinothalamic neurons were serially sectioned and examined at selected intervals at the electron microscopic level. Electron microscopic study revealed that axonal boutons directly contacted the somata and/or dendrites of lamina I, IV, and V spinothalamic tract neurons. All of the profiles apposing one of the retrogradely labeled lamina I spinothalamic tract neurons were categorized from eight planes of section spaced at 1-μm intervals. Of the 305 profiles counted that were adjacent to this soma, 17 (5.6{\%}) stained positively for dopamine-beta-hydroxylase. Of these 17 appositions, three were followed in serial sections to confirm that they had synaptic thickenings and alignment of vesicles along the membrane contacting the spinothalamic tract soma. Catecholaminergic boutons were observed apposing the somata and dendrites of intracellularly filled STT cells characterized as high threshold and wide dynamic range neurons. These observations clearly indicate a direct innervation of spinothalamic tract neurons by catecholaminergic neurons, providing anatomical data to support previous physiological findings demonstrating that catecholamines modulate nociceptive transmission.",
    keywords = "catecholamines, dopamine-beta-hydroxylase, electron microscopy, immunocytochemistry, norepinephrine, spinal cord",
    author = "Westlund, {K. N.} and Carlton, {S. M.} and D. Zhang and Willis, {W. D.}",
    year = "1990",
    language = "English (US)",
    volume = "299",
    pages = "178--186",
    journal = "Journal of Comparative Neurology",
    issn = "0021-9967",
    publisher = "Wiley-Liss Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Direct catecholaminergic innervation of primate spinothalamic tract neurons

    AU - Westlund, K. N.

    AU - Carlton, S. M.

    AU - Zhang, D.

    AU - Willis, W. D.

    PY - 1990

    Y1 - 1990

    N2 - Catecholaminergic axonal varicosities identified by immunocytochemical staining for dopamine-beta-hydroxylase were observed at the light microscopic level apposing the somata of retrogradely labeled spinothalamic tract neurons in the monkey spinal cord. Three retrogradely labeled and two intracellularly labeled spinothalamic neurons were serially sectioned and examined at selected intervals at the electron microscopic level. Electron microscopic study revealed that axonal boutons directly contacted the somata and/or dendrites of lamina I, IV, and V spinothalamic tract neurons. All of the profiles apposing one of the retrogradely labeled lamina I spinothalamic tract neurons were categorized from eight planes of section spaced at 1-μm intervals. Of the 305 profiles counted that were adjacent to this soma, 17 (5.6%) stained positively for dopamine-beta-hydroxylase. Of these 17 appositions, three were followed in serial sections to confirm that they had synaptic thickenings and alignment of vesicles along the membrane contacting the spinothalamic tract soma. Catecholaminergic boutons were observed apposing the somata and dendrites of intracellularly filled STT cells characterized as high threshold and wide dynamic range neurons. These observations clearly indicate a direct innervation of spinothalamic tract neurons by catecholaminergic neurons, providing anatomical data to support previous physiological findings demonstrating that catecholamines modulate nociceptive transmission.

    AB - Catecholaminergic axonal varicosities identified by immunocytochemical staining for dopamine-beta-hydroxylase were observed at the light microscopic level apposing the somata of retrogradely labeled spinothalamic tract neurons in the monkey spinal cord. Three retrogradely labeled and two intracellularly labeled spinothalamic neurons were serially sectioned and examined at selected intervals at the electron microscopic level. Electron microscopic study revealed that axonal boutons directly contacted the somata and/or dendrites of lamina I, IV, and V spinothalamic tract neurons. All of the profiles apposing one of the retrogradely labeled lamina I spinothalamic tract neurons were categorized from eight planes of section spaced at 1-μm intervals. Of the 305 profiles counted that were adjacent to this soma, 17 (5.6%) stained positively for dopamine-beta-hydroxylase. Of these 17 appositions, three were followed in serial sections to confirm that they had synaptic thickenings and alignment of vesicles along the membrane contacting the spinothalamic tract soma. Catecholaminergic boutons were observed apposing the somata and dendrites of intracellularly filled STT cells characterized as high threshold and wide dynamic range neurons. These observations clearly indicate a direct innervation of spinothalamic tract neurons by catecholaminergic neurons, providing anatomical data to support previous physiological findings demonstrating that catecholamines modulate nociceptive transmission.

    KW - catecholamines

    KW - dopamine-beta-hydroxylase

    KW - electron microscopy

    KW - immunocytochemistry

    KW - norepinephrine

    KW - spinal cord

    UR - http://www.scopus.com/inward/record.url?scp=0025103040&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0025103040&partnerID=8YFLogxK

    M3 - Article

    C2 - 2229478

    AN - SCOPUS:0025103040

    VL - 299

    SP - 178

    EP - 186

    JO - Journal of Comparative Neurology

    JF - Journal of Comparative Neurology

    SN - 0021-9967

    IS - 2

    ER -