Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons

Ping Wu, Patricia Price, Bin Du, William C. Hatch, Ernest F. Terwilliger

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A new in vitro system comprising a pure population of neurons, human NT cells, was used to characterize the direct neurotoxic effect of HIV-1 envelope protein gp120. Cytotoxicity was monitored by a quantitative assay after exposure to recombinant gp120 in the presence or absence of other reagents. Treatment of mature NT neurons with various doses of gp120 for 24 h caused a decrease of up to 27% in the number of viable cells. This neurotoxicity was abolished by co-treatment with either D-2-amino-5- phosphonopentanoic acid (APV), MK801 or nimodipine, or by culturing cells in a Ca 2+-free environment. Taken together, these data indicate that gp120 exerts a direct neurotoxic effect by acting through NMDA receptors and Ca 2+ channels.

Original languageEnglish (US)
Pages (from-to)1045-1049
Number of pages5
JournalNeuroReport
Volume7
Issue number5
StatePublished - 1996
Externally publishedYes

Fingerprint

2-Amino-5-phosphonovalerate
Neurons
Nimodipine
N-Methyl-D-Aspartate Receptors
Cell Count
Population
Human immunodeficiency virus 1 gp120 protein
In Vitro Techniques

Keywords

  • Gp120 protein
  • HIV
  • Human NT neuron
  • Neurotoxicity
  • NMDA receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wu, P., Price, P., Du, B., Hatch, W. C., & Terwilliger, E. F. (1996). Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons. NeuroReport, 7(5), 1045-1049.

Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons. / Wu, Ping; Price, Patricia; Du, Bin; Hatch, William C.; Terwilliger, Ernest F.

In: NeuroReport, Vol. 7, No. 5, 1996, p. 1045-1049.

Research output: Contribution to journalArticle

Wu, P, Price, P, Du, B, Hatch, WC & Terwilliger, EF 1996, 'Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons', NeuroReport, vol. 7, no. 5, pp. 1045-1049.
Wu P, Price P, Du B, Hatch WC, Terwilliger EF. Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons. NeuroReport. 1996;7(5):1045-1049.
Wu, Ping ; Price, Patricia ; Du, Bin ; Hatch, William C. ; Terwilliger, Ernest F. / Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons. In: NeuroReport. 1996 ; Vol. 7, No. 5. pp. 1045-1049.
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