Direct delivery of low-dose 7-Nitroindazole into the bronchial artery attenuates pulmonary pathophysiology after smoke inhalation and burn injury in an ovine model

Atsumori Hamahata, Perenlei Enkhbaatar, Matthias Lange, Robert A. Cox, Hal K. Hawkins, Hiroyuki Sakurai, Lillian D. Traber, Daniel L. Traber

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bronchial circulation plays a critical role in the pathophysiology of burn and smoke inhalation-induced acute lung injury. A 10-fold increase in bronchial blood flow is associated with excessive production of nitric oxide (NO) following smoke inhalation and cutaneous burn. Because an increased release of neuropeptides from the airway has been implicated in smoke inhalation injury, we hypothesized that direct delivery into the bronchial artery of low-dose 7-nitroindazole (7-NI), a specific neuronal NO synthase inhibitor, would attenuate smoke/burn-induced acute lung injury. Eighteen adult female sheep were instrumented for chronic hemodynamic monitoring 5 to 7 days before the injury. The bronchial artery was cannulated via intercostal thoracotomy, while blood flow was preserved. Acute lung injury was induced by 40% total body surface area third-degree cutaneous burn and smoke inhalation (48 breaths of cotton smoke, <40°C) under deep anesthesia. Following injury, animals (35.4 ± 1.1 kg) were divided into three groups: (a) 7-NI group: 1 h after injury, 7-NI (0.01 mg • kg • h, 2 mL • h) was continuously infused into the bronchial artery, n = 6; (b) control group: 1 h after injury, same amount of saline was injected into the bronchial artery, n = 6; (c) sham group: no injury, no treatment, same operation and anesthesia, n = 6. After injury, all animals were ventilated and fluid resuscitated according to an established protocol. The experiment was conducted for 24 h. Injury induced severe pulmonary dysfunction, which was associated with increases in lung edema formation, airway obstruction, malondialdehyde, and nitrate/nitrite. 7-Nitroindazole injection into the bronchial artery reduced the degree of lung edema formation and improved pulmonary gas exchange. The increase in malondialdehyde and nitrate/nitrite in lung tissue was attenuated by treatment. Our data strongly suggest that local airway production of NO contributes to pulmonary dysfunction following smoke inhalation and burn injury. Most mechanisms that drive this pathophysiology reside in the airway.

Original languageEnglish (US)
Pages (from-to)575-579
Number of pages5
JournalShock
Volume36
Issue number6
DOIs
StatePublished - Dec 2011

Fingerprint

Inhalation Burns
Smoke Inhalation Injury
Bronchial Arteries
Sheep
Smoke
Lung
Wounds and Injuries
Acute Lung Injury
Nitrites
Malondialdehyde
Nitrates
Edema
Nitric Oxide
Anesthesia
Pulmonary Gas Exchange
Nitric Oxide Synthase Type I
Skin
Body Surface Area
Airway Obstruction
Thoracotomy

Keywords

  • 7-nitroindazole
  • bronchial artery
  • Burn
  • neuronal NOS
  • smoke inhalation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Direct delivery of low-dose 7-Nitroindazole into the bronchial artery attenuates pulmonary pathophysiology after smoke inhalation and burn injury in an ovine model. / Hamahata, Atsumori; Enkhbaatar, Perenlei; Lange, Matthias; Cox, Robert A.; Hawkins, Hal K.; Sakurai, Hiroyuki; Traber, Lillian D.; Traber, Daniel L.

In: Shock, Vol. 36, No. 6, 12.2011, p. 575-579.

Research output: Contribution to journalArticle

Hamahata, Atsumori ; Enkhbaatar, Perenlei ; Lange, Matthias ; Cox, Robert A. ; Hawkins, Hal K. ; Sakurai, Hiroyuki ; Traber, Lillian D. ; Traber, Daniel L. / Direct delivery of low-dose 7-Nitroindazole into the bronchial artery attenuates pulmonary pathophysiology after smoke inhalation and burn injury in an ovine model. In: Shock. 2011 ; Vol. 36, No. 6. pp. 575-579.
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