Direct detection of lysine side chain NH3 + in protein-heparin complexes using NMR spectroscopy

Krishna Mohan Sepuru, Junji Iwahara, Krishna Rajarathnam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two NMR observables, the NζH3 + peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

Original languageEnglish (US)
Pages (from-to)635-638
Number of pages4
JournalAnalyst
Volume143
Issue number3
DOIs
StatePublished - Feb 7 2018

Fingerprint

Chemical shift
Nuclear magnetic resonance spectroscopy
Lysine
nuclear magnetic resonance
Heparin
Magnetic Resonance Spectroscopy
spectroscopy
Proteins
protein
probe
Nuclear magnetic resonance
chemical
detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Direct detection of lysine side chain NH3 + in protein-heparin complexes using NMR spectroscopy. / Sepuru, Krishna Mohan; Iwahara, Junji; Rajarathnam, Krishna.

In: Analyst, Vol. 143, No. 3, 07.02.2018, p. 635-638.

Research output: Contribution to journalArticle

@article{7e7c6f90f86d4820a21c2831044d91cd,
title = "Direct detection of lysine side chain NH3 + in protein-heparin complexes using NMR spectroscopy",
abstract = "Two NMR observables, the NζH3 + peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.",
author = "Sepuru, {Krishna Mohan} and Junji Iwahara and Krishna Rajarathnam",
year = "2018",
month = "2",
day = "7",
doi = "10.1039/c7an01406f",
language = "English (US)",
volume = "143",
pages = "635--638",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "3",

}

TY - JOUR

T1 - Direct detection of lysine side chain NH3 + in protein-heparin complexes using NMR spectroscopy

AU - Sepuru, Krishna Mohan

AU - Iwahara, Junji

AU - Rajarathnam, Krishna

PY - 2018/2/7

Y1 - 2018/2/7

N2 - Two NMR observables, the NζH3 + peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

AB - Two NMR observables, the NζH3 + peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

UR - http://www.scopus.com/inward/record.url?scp=85041342226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041342226&partnerID=8YFLogxK

U2 - 10.1039/c7an01406f

DO - 10.1039/c7an01406f

M3 - Article

VL - 143

SP - 635

EP - 638

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 3

ER -