Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy

Krishna Mohan Sepuru, Junji Iwahara, Krishna Rajarathnam

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Two NMR observables, the NζH3+ peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

Original languageEnglish (US)
Pages (from-to)635-638
Number of pages4
Issue number3
StatePublished - Feb 7 2018

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


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