Abstract
Two NMR observables, the NζH3+ peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 635-638 |
| Number of pages | 4 |
| Journal | Analyst |
| Volume | 143 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 7 2018 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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