Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations

Joyce Grant Lewis; Gwong Jen Chang; Robert S. Lanciotti; Dennis W. Trent

doi:10.1016/0166-0934(92)90165-A

Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations

Joyce Grant Lewis, Gwong Jen Chang, Robert S. Lanciotti, Dennis W. Trent

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

Original language	English (US)
Pages (from-to)	11-23
Number of pages	13
Journal	Journal of Virological Methods
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(92)90165-A
State	Published - Jul 1992
Externally published	Yes

Keywords

(PCR)
Dengue
Polymerase chain reaction
Sequencing

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(92)90165-A

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title = "Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations",

abstract = "The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.",

keywords = "(PCR), Dengue, Polymerase chain reaction, Sequencing",

author = "Lewis, {Joyce Grant} and Chang, {Gwong Jen} and Lanciotti, {Robert S.} and Trent, {Dennis W.}",

year = "1992",

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doi = "10.1016/0166-0934(92)90165-A",

language = "English (US)",

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T1 - Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations

AU - Lewis, Joyce Grant

AU - Chang, Gwong Jen

AU - Lanciotti, Robert S.

AU - Trent, Dennis W.

PY - 1992/7

Y1 - 1992/7

N2 - The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

AB - The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

KW - (PCR)

KW - Dengue

KW - Polymerase chain reaction

KW - Sequencing

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations

Abstract

Keywords

ASJC Scopus subject areas

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