Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations

Joyce Grant Lewis, Gwong Jen Chang, Robert S. Lanciotti, Dennis W. Trent

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

Original languageEnglish (US)
Pages (from-to)11-23
Number of pages13
JournalJournal of Virological Methods
Volume38
Issue number1
DOIs
StatePublished - 1992

Fingerprint

Flavivirus
Dengue
Genome
Polymerase Chain Reaction
Dengue Virus
Molecular Cloning
Epidemiologic Studies
Complementary DNA
RNA
Viruses
DNA
Genes
Serogroup

Keywords

  • (PCR)
  • Dengue
  • Polymerase chain reaction
  • Sequencing

ASJC Scopus subject areas

  • Virology

Cite this

Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations. / Lewis, Joyce Grant; Chang, Gwong Jen; Lanciotti, Robert S.; Trent, Dennis W.

In: Journal of Virological Methods, Vol. 38, No. 1, 1992, p. 11-23.

Research output: Contribution to journalArticle

Lewis, Joyce Grant ; Chang, Gwong Jen ; Lanciotti, Robert S. ; Trent, Dennis W. / Direct sequencing of large flavivirus PCR products for analysis of genome variation and molecular epidemiological investigations. In: Journal of Virological Methods. 1992 ; Vol. 38, No. 1. pp. 11-23.
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