Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong, Jairam R. Lingappa, Qi Chen, Bo Shu, Ashley C. LaMonte, Byron T. Cook, Charryse Birge, Wang Chern Shur-Wern, Xin Liu, Renee Galloway, Quynh Mai Le, Fu Ng Wai, Jyh Yuan Yang, Jagdish Butany, James A. Comer, Stephan S. Monroe, Suzanne R. Beard, Thomas Ksiazek, Dean Erdman, Paul A. Rota & 2 others Mark A. Pallansch, Larry J. Anderson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

Original languageEnglish (US)
Pages (from-to)1127-1131
Number of pages5
JournalJournal of Infectious Diseases
Volume190
Issue number6
DOIs
StatePublished - Sep 15 2004
Externally publishedYes

Fingerprint

SARS Virus
Severe Acute Respiratory Syndrome
Coronavirus
Genes
Cell Culture Techniques
Viruses
Nucleic Acids
Nucleotides
Genome

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Tong, S., Lingappa, J. R., Chen, Q., Shu, B., LaMonte, A. C., Cook, B. T., ... Anderson, L. J. (2004). Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation. Journal of Infectious Diseases, 190(6), 1127-1131. https://doi.org/10.1086/422849

Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation. / Tong, Suxiang; Lingappa, Jairam R.; Chen, Qi; Shu, Bo; LaMonte, Ashley C.; Cook, Byron T.; Birge, Charryse; Shur-Wern, Wang Chern; Liu, Xin; Galloway, Renee; Le, Quynh Mai; Wai, Fu Ng; Yang, Jyh Yuan; Butany, Jagdish; Comer, James A.; Monroe, Stephan S.; Beard, Suzanne R.; Ksiazek, Thomas; Erdman, Dean; Rota, Paul A.; Pallansch, Mark A.; Anderson, Larry J.

In: Journal of Infectious Diseases, Vol. 190, No. 6, 15.09.2004, p. 1127-1131.

Research output: Contribution to journalArticle

Tong, S, Lingappa, JR, Chen, Q, Shu, B, LaMonte, AC, Cook, BT, Birge, C, Shur-Wern, WC, Liu, X, Galloway, R, Le, QM, Wai, FN, Yang, JY, Butany, J, Comer, JA, Monroe, SS, Beard, SR, Ksiazek, T, Erdman, D, Rota, PA, Pallansch, MA & Anderson, LJ 2004, 'Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation', Journal of Infectious Diseases, vol. 190, no. 6, pp. 1127-1131. https://doi.org/10.1086/422849
Tong, Suxiang ; Lingappa, Jairam R. ; Chen, Qi ; Shu, Bo ; LaMonte, Ashley C. ; Cook, Byron T. ; Birge, Charryse ; Shur-Wern, Wang Chern ; Liu, Xin ; Galloway, Renee ; Le, Quynh Mai ; Wai, Fu Ng ; Yang, Jyh Yuan ; Butany, Jagdish ; Comer, James A. ; Monroe, Stephan S. ; Beard, Suzanne R. ; Ksiazek, Thomas ; Erdman, Dean ; Rota, Paul A. ; Pallansch, Mark A. ; Anderson, Larry J. / Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation. In: Journal of Infectious Diseases. 2004 ; Vol. 190, No. 6. pp. 1127-1131.
@article{60224a942ca04422b0f3683ba4059e47,
title = "Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation",
abstract = "Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.",
author = "Suxiang Tong and Lingappa, {Jairam R.} and Qi Chen and Bo Shu and LaMonte, {Ashley C.} and Cook, {Byron T.} and Charryse Birge and Shur-Wern, {Wang Chern} and Xin Liu and Renee Galloway and Le, {Quynh Mai} and Wai, {Fu Ng} and Yang, {Jyh Yuan} and Jagdish Butany and Comer, {James A.} and Monroe, {Stephan S.} and Beard, {Suzanne R.} and Thomas Ksiazek and Dean Erdman and Rota, {Paul A.} and Pallansch, {Mark A.} and Anderson, {Larry J.}",
year = "2004",
month = "9",
day = "15",
doi = "10.1086/422849",
language = "English (US)",
volume = "190",
pages = "1127--1131",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

AU - Tong, Suxiang

AU - Lingappa, Jairam R.

AU - Chen, Qi

AU - Shu, Bo

AU - LaMonte, Ashley C.

AU - Cook, Byron T.

AU - Birge, Charryse

AU - Shur-Wern, Wang Chern

AU - Liu, Xin

AU - Galloway, Renee

AU - Le, Quynh Mai

AU - Wai, Fu Ng

AU - Yang, Jyh Yuan

AU - Butany, Jagdish

AU - Comer, James A.

AU - Monroe, Stephan S.

AU - Beard, Suzanne R.

AU - Ksiazek, Thomas

AU - Erdman, Dean

AU - Rota, Paul A.

AU - Pallansch, Mark A.

AU - Anderson, Larry J.

PY - 2004/9/15

Y1 - 2004/9/15

N2 - Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

AB - Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients-1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

UR - http://www.scopus.com/inward/record.url?scp=4444370074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444370074&partnerID=8YFLogxK

U2 - 10.1086/422849

DO - 10.1086/422849

M3 - Article

VL - 190

SP - 1127

EP - 1131

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 6

ER -