Directed discovery of bivalent peptide ligands to an SH3 domain

Monique Ferguson, Xiuzhen Fan, Munia Mukherjee, Jinquan Luo, Raza Khan, Josephine C. Ferreon, Vincent J. Hilser, Robert E. Shope, Robert O. Fox

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The Caenorhabditis elegans SEM-5 SH3 domains recognize proline-rich peptide segments with modest affinity. We developed a bivalent peptide ligand that contains a naturally occurring proline-rich binding sequence, tethered by a glycine linker to a disulfide-closed loop segment containing six variable residues. The glycine linker allows the loop segment to explore regions of greatest diversity in sequence and structure of the SH3 domain: the RT and n-Src loops. The bivalent ligand was optimized using phage display, leading to a peptide (PP-G4-L) with 1000-fold increased affinity for the SEM-5 C-terminal SH3 domain over that of a natural ligand. NMR analysis of the complex confirms that the peptide loop segment is targeted to the RT and n-Src loops and parts of the β-sheet scaffold of this SH3 domain. This binding region is comparable to that targeted by a natural non-PXXP peptide to the p67phox SH3 domain, a region not known to be targeted in the Grb2 SH3 domain family. PP-G4-L may aid in the discovery of additional binding partners of Grb2 family SH3 domains.

Original languageEnglish (US)
Pages (from-to)626-632
Number of pages7
JournalProtein Science
Volume13
Issue number3
DOIs
StatePublished - Mar 2004

Fingerprint

src Homology Domains
Ligands
Peptides
Proline
Glycine
Scanning electron microscopy
Bacteriophages
Scaffolds
Disulfides
Caenorhabditis elegans
Display devices
Nuclear magnetic resonance

Keywords

  • Bivalent ligand
  • Combinatorial library
  • NMR spectroscopy
  • Non-PXXP binding site
  • Peptide ligand
  • Phage display
  • SH3 domain
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ferguson, M., Fan, X., Mukherjee, M., Luo, J., Khan, R., Ferreon, J. C., ... Fox, R. O. (2004). Directed discovery of bivalent peptide ligands to an SH3 domain. Protein Science, 13(3), 626-632. https://doi.org/10.1110/ps.03470504

Directed discovery of bivalent peptide ligands to an SH3 domain. / Ferguson, Monique; Fan, Xiuzhen; Mukherjee, Munia; Luo, Jinquan; Khan, Raza; Ferreon, Josephine C.; Hilser, Vincent J.; Shope, Robert E.; Fox, Robert O.

In: Protein Science, Vol. 13, No. 3, 03.2004, p. 626-632.

Research output: Contribution to journalArticle

Ferguson, M, Fan, X, Mukherjee, M, Luo, J, Khan, R, Ferreon, JC, Hilser, VJ, Shope, RE & Fox, RO 2004, 'Directed discovery of bivalent peptide ligands to an SH3 domain', Protein Science, vol. 13, no. 3, pp. 626-632. https://doi.org/10.1110/ps.03470504
Ferguson M, Fan X, Mukherjee M, Luo J, Khan R, Ferreon JC et al. Directed discovery of bivalent peptide ligands to an SH3 domain. Protein Science. 2004 Mar;13(3):626-632. https://doi.org/10.1110/ps.03470504
Ferguson, Monique ; Fan, Xiuzhen ; Mukherjee, Munia ; Luo, Jinquan ; Khan, Raza ; Ferreon, Josephine C. ; Hilser, Vincent J. ; Shope, Robert E. ; Fox, Robert O. / Directed discovery of bivalent peptide ligands to an SH3 domain. In: Protein Science. 2004 ; Vol. 13, No. 3. pp. 626-632.
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