Directional genomic hybridization

Inversions as a potential biodosimeter for retrospective radiation exposure

F. Andrew Ray, Erin Robinson, Miles McKenna, Megumi Hada, Kerry George, Francis Cucinotta, Edwin H. Goodwin, Joel S. Bedford, Susan M. Bailey, Michael Cornforth

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Chromosome aberrations in blood lymphocytes provide a useful measure of past exposure to ionizing radiation. Despite the widespread and successful use of the dicentric assay for retrospective biodosimetry, the approach suffers substantial drawbacks, including the fact that dicentrics in circulating blood have a rather short halflife (roughly 1-2 years by most estimates). So-called symmetrical aberrations such as translocations are far more stable in that regard, but their high background frequency, which increases with age, also makes them less than ideal for biodosimetry. We developed a cytogenetic assay for potential use in retrospective biodosimetry that is based on the detection of chromosomal inversions, another symmetrical aberration whose transmissibility (stability) is also ostensibly high. Many of the well-known difficulties associated with inversion detection were circumvented through the use of directional genomic hybridization, a method of molecular cytogenetics that is less labor intensive and better able to detect small chromosomal inversions than other currently available approaches. Here, we report the dose-dependent induction of inversions following exposure to radiations with vastly different ionization densities [i.e., linear energy transfer (LET)]. Our results show a dramatic dose-dependent difference in the yields of inversions induced by low-LET gamma rays, as compared to more damaging high-LET charged particles similar to those encountered in deep space.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalRadiation and Environmental Biophysics
Volume53
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Nucleic Acid Hybridization
Linear Energy Transfer
radiation exposure
radiation dosage
genomics
linear energy transfer (LET)
inversions
Cytogenetics
cytogenetics
Gamma Rays
blood
aberration
Ionizing Radiation
Chromosome Aberrations
Half-Life
assay
energy
chromosome aberrations
dosage
deep space

Keywords

  • Biodosimetry
  • Chromosome inversions
  • DGH
  • Directional genomic hybridization
  • FISH
  • Ionizing radiation
  • Strand-specific hybridization

ASJC Scopus subject areas

  • Biophysics
  • Environmental Science(all)
  • Radiation
  • Medicine(all)

Cite this

Directional genomic hybridization : Inversions as a potential biodosimeter for retrospective radiation exposure. / Ray, F. Andrew; Robinson, Erin; McKenna, Miles; Hada, Megumi; George, Kerry; Cucinotta, Francis; Goodwin, Edwin H.; Bedford, Joel S.; Bailey, Susan M.; Cornforth, Michael.

In: Radiation and Environmental Biophysics, Vol. 53, No. 2, 2014, p. 255-263.

Research output: Contribution to journalArticle

Ray, FA, Robinson, E, McKenna, M, Hada, M, George, K, Cucinotta, F, Goodwin, EH, Bedford, JS, Bailey, SM & Cornforth, M 2014, 'Directional genomic hybridization: Inversions as a potential biodosimeter for retrospective radiation exposure', Radiation and Environmental Biophysics, vol. 53, no. 2, pp. 255-263. https://doi.org/10.1007/s00411-014-0513-1
Ray, F. Andrew ; Robinson, Erin ; McKenna, Miles ; Hada, Megumi ; George, Kerry ; Cucinotta, Francis ; Goodwin, Edwin H. ; Bedford, Joel S. ; Bailey, Susan M. ; Cornforth, Michael. / Directional genomic hybridization : Inversions as a potential biodosimeter for retrospective radiation exposure. In: Radiation and Environmental Biophysics. 2014 ; Vol. 53, No. 2. pp. 255-263.
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