Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs

Shinji Makino, L. H. Soe, C. K. Shieh, M. M C Lai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Coronavirus mRNA is synthesized by a discontinuous transcription process, which involves a free leader RNA species. As a result, each virus-specific mRNA contains an identical leader RNA derived from the 5' end of the genomic RNA. In this study, we demonstrate by primer extension studies that the leader-fusion sites on a given species of coronavirus subgenomic mRNA are heterogeneous. The heterogeneity was due to variation in the number of pentanucleotide (UCUAA) repeats present at the leader fusion site. This pentanucleotide repeat region was complementary between the free leader RNA and the transcription start sites on the template RNA. This result suggests that the discontinuous transcription of coronavirus mRNAs occurs within the complementary sequences localized in two different RNA segments and that RNA joining occurs at variable sites.

Original languageEnglish (US)
Pages (from-to)3870-3873
Number of pages4
JournalJournal of Virology
Volume62
Issue number10
StatePublished - 1988
Externally publishedYes

Fingerprint

Coronavirus
Coronavirinae
transcription (genetics)
RNA
Messenger RNA
Microsatellite Repeats
microsatellite repeats
Transcription Initiation Site
Viruses
genomics
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs. / Makino, Shinji; Soe, L. H.; Shieh, C. K.; Lai, M. M C.

In: Journal of Virology, Vol. 62, No. 10, 1988, p. 3870-3873.

Research output: Contribution to journalArticle

Makino, Shinji ; Soe, L. H. ; Shieh, C. K. ; Lai, M. M C. / Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs. In: Journal of Virology. 1988 ; Vol. 62, No. 10. pp. 3870-3873.
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