@article{8b9e65af6dd24103b5ce82223f14477f,
title = "Discovery and characterization of a novel CCND1/MRCK gene fusion in mantle cell lymphoma",
abstract = "The t(11;14) translocation resulting in constitutive cyclin D1 expression is an early event in mantle cell lymphoma (MCL) transformation. Patients with a highly proliferative phenotype produce cyclin D1 transcripts with truncated 3'UTRs that evade miRNA regulation. Here, we report the recurrence of a novel gene fusion in MCL cell lines and MCL patient isolates that consists of the full protein coding region of cyclin D1 (CCND1) and a 3'UTR consisting of sequences from both the CCND1 3'UTR and myotonic dystrophy kinase-related Cdc42-binding kinase's (MRCK) intron one. The resulting CCND1/MRCK mRNA is resistant to CCND1-targeted miRNA regulation, and targeting the MRCK region of the chimeric 3'UTR with siRNA results in decreased CCND1 levels.",
keywords = "3'UTR, Alternative polyadenylation, Cyclin D1, Gene fusion, Mantle cell lymphoma, MiRNA",
author = "Masamha, {Chioniso Patience} and Albrecht, {Todd R.} and Wagner, {Eric J.}",
note = "Funding Information: Many thanks to Dr. Todd Hrubey at Butler University for his critical reading of the manuscript. This work was funded by the National Institute of Health (EJW CA167752 and CA166274). CPM acknowledges a Department of Defense Visionary Postdoctoral Fellowship (W81XWH-12-1-0218). Patient DNA samples were obtained from Indiana Biobank in Indiana. This project was supported, in part, with support from the Indiana Clinical and Translational Sciences Institute funded, in part by Grant Number UL1TR001108 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award and the National Center for Research Resources, Construction grant number RR020128 and the Lilly Endowment. We thank the contributors who collected samples and/or data used in this study, as well as subjects whose help and participation made this work possible. Publisher Copyright: {\textcopyright} 2016 Masamha et al.",
year = "2016",
doi = "10.1186/s13045-016-0260-7",
language = "English (US)",
volume = "9",
journal = "Journal of Hematology and Oncology",
issn = "1756-8722",
publisher = "BioMed Central",
number = "1",
}