Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women

The PAPR Study Consortium

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Development of effective prevention and treatment strategies for pre-eclampsia is limited by the lack of accurate methods for identification of at-risk pregnancies. We performed small RNA sequencing (RNA-seq) of maternal serum extracellular RNAs (exRNAs) to discover and verify microRNAs (miRNAs) differentially expressed in patients who later developed pre-eclampsia. Sera collected from 73 pre-eclampsia cases and 139 controls between 17 and 28 weeks gestational age (GA), divided into separate discovery and verification cohorts, are analyzed by small RNA-seq. Discovery and verification of univariate and bivariate miRNA biomarkers reveal that bivariate biomarkers verify at a markedly higher rate than univariate biomarkers. The majority of verified biomarkers contain miR-155-5p, which has been reported to mediate the pre-eclampsia-associated repression of endothelial nitric oxide synthase (eNOS) by tumor necrosis factor alpha (TNF-α). Deconvolution analysis reveals that several verified miRNA biomarkers come from the placenta and are likely carried by placenta-specific extracellular vesicles.

Original languageEnglish (US)
Article number100013
JournalCell Reports Medicine
Volume1
Issue number2
DOIs
StatePublished - May 19 2020
Externally publishedYes

Keywords

  • biomarkers
  • bivariate
  • extracellular RNA
  • extracellular vesicle
  • maternal serum
  • miR-155-5p
  • miRNA
  • placenta
  • pre-eclampsia
  • small RNA-seq

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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