Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Platform

Michal Gazi, Erika Caro-Gomez, Yenny Goez, Maria A. Cespedes, Marylin Hidalgo, Paula Correa, Gustavo Valbuena

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rickettsia prowazekii has been tested for biological warfare due to the high mortality that it produces after aerosol transmission of very low numbers of rickettsiae. Epidemic typhus, the infection caused by these obligately intracellular bacteria, continues to be a threat because it is difficult to diagnose due to initial non-specific symptoms and the lack of commercial diagnostic tests that are sensitive and specific during the initial clinical presentation. A vaccine to prevent epidemic typhus would constitute an effective deterrent to the weaponization of R. prowazekii; however, an effective and safe vaccine is not currently available. Due to the cytoplasmic niche of Rickettsia, CD8+ T-cells are critical effectors of immunity; however, the identification of antigens recognized by these cells has not been systematically addressed. To help close this gap, we designed an antigen discovery strategy that uses cell-based vaccination with antigen presenting cells expressing microbe's proteins targeted to the MHC class I presentation pathway. We report the use of this method to discover a protective T-cell rickettsial antigen, RP884, among a test subset of rickettsial proteins.

Original languageEnglish (US)
Article numbere76253
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 16 2013

Fingerprint

Rickettsia prowazekii
CD8 Antigens
T-cells
typhus
Epidemic Louse-Borne Typhus
Rickettsia
Screening
T-lymphocytes
screening
antigens
T-Lymphocytes
Antigens
Biological warfare
Biological Warfare
Vaccines
vaccines
antigen-presenting cells
Antigen-Presenting Cells
aerosols
Aerosols

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gazi, M., Caro-Gomez, E., Goez, Y., Cespedes, M. A., Hidalgo, M., Correa, P., & Valbuena, G. (2013). Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Platform. PLoS One, 8(10), [e76253]. https://doi.org/10.1371/journal.pone.0076253

Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Platform. / Gazi, Michal; Caro-Gomez, Erika; Goez, Yenny; Cespedes, Maria A.; Hidalgo, Marylin; Correa, Paula; Valbuena, Gustavo.

In: PLoS One, Vol. 8, No. 10, e76253, 16.10.2013.

Research output: Contribution to journalArticle

Gazi, M, Caro-Gomez, E, Goez, Y, Cespedes, MA, Hidalgo, M, Correa, P & Valbuena, G 2013, 'Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Platform', PLoS One, vol. 8, no. 10, e76253. https://doi.org/10.1371/journal.pone.0076253
Gazi, Michal ; Caro-Gomez, Erika ; Goez, Yenny ; Cespedes, Maria A. ; Hidalgo, Marylin ; Correa, Paula ; Valbuena, Gustavo. / Discovery of a Protective Rickettsia prowazekii Antigen Recognized by CD8+ T Cells, RP884, Using an In Vivo Screening Platform. In: PLoS One. 2013 ; Vol. 8, No. 10.
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