Discovery strategies for proteomic profiling of airway diseases

John E. Wiktorowicz, Kizhake V. Soman, Anthony Haag

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Discovery proteomics is defined here as the unbiased (untargeted) differential investigation of protein abundances from biological material resulting from some biological or physical challenge. In this review, we will examine the latest approaches and strategies for discovery investigations of airway disease. We will discuss general and specific sampling strategies, sample preparation, differential separations and quantification-with special attention to posttranslational modifications that are particularly relevant to oxidative stress response of airway tissues, statistical analysis and tools, mass spectrometric identification, and finally bioinformatics tools for pathway analysis.

    Original languageEnglish (US)
    Pages (from-to)97-110
    Number of pages14
    JournalCurrent Proteomics
    Volume8
    Issue number2
    StatePublished - Jul 2011

    Fingerprint

    Post Translational Protein Processing
    Computational Biology
    Proteomics
    Oxidative Stress
    Oxidative stress
    Bioinformatics
    Biological materials
    Statistical methods
    Proteins
    Tissue
    Sampling

    Keywords

    • Airway disease
    • Bioinformatics tools
    • Gel analysis
    • Mass spectrometry
    • Proteomics discovery
    • Sample fractionation
    • Sample preparation
    • Two dimensional gel electrophoresis

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    Wiktorowicz, J. E., Soman, K. V., & Haag, A. (2011). Discovery strategies for proteomic profiling of airway diseases. Current Proteomics, 8(2), 97-110.

    Discovery strategies for proteomic profiling of airway diseases. / Wiktorowicz, John E.; Soman, Kizhake V.; Haag, Anthony.

    In: Current Proteomics, Vol. 8, No. 2, 07.2011, p. 97-110.

    Research output: Contribution to journalArticle

    Wiktorowicz, JE, Soman, KV & Haag, A 2011, 'Discovery strategies for proteomic profiling of airway diseases', Current Proteomics, vol. 8, no. 2, pp. 97-110.
    Wiktorowicz JE, Soman KV, Haag A. Discovery strategies for proteomic profiling of airway diseases. Current Proteomics. 2011 Jul;8(2):97-110.
    Wiktorowicz, John E. ; Soman, Kizhake V. ; Haag, Anthony. / Discovery strategies for proteomic profiling of airway diseases. In: Current Proteomics. 2011 ; Vol. 8, No. 2. pp. 97-110.
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