Discovery strategies for proteomic profiling of airway diseases

John E. Wiktorowicz, Kizhake V. Soman, Anthony Haag

    Research output: Contribution to journalArticle

    2 Scopus citations

    Abstract

    Discovery proteomics is defined here as the unbiased (untargeted) differential investigation of protein abundances from biological material resulting from some biological or physical challenge. In this review, we will examine the latest approaches and strategies for discovery investigations of airway disease. We will discuss general and specific sampling strategies, sample preparation, differential separations and quantification-with special attention to posttranslational modifications that are particularly relevant to oxidative stress response of airway tissues, statistical analysis and tools, mass spectrometric identification, and finally bioinformatics tools for pathway analysis.

    Original languageEnglish (US)
    Pages (from-to)97-110
    Number of pages14
    JournalCurrent Proteomics
    Volume8
    Issue number2
    StatePublished - Jul 2011

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    Keywords

    • Airway disease
    • Bioinformatics tools
    • Gel analysis
    • Mass spectrometry
    • Proteomics discovery
    • Sample fractionation
    • Sample preparation
    • Two dimensional gel electrophoresis

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    Wiktorowicz, J. E., Soman, K. V., & Haag, A. (2011). Discovery strategies for proteomic profiling of airway diseases. Current Proteomics, 8(2), 97-110.