Distinct influences of nerve growth factor and a central cholinergic trophic factor on medial septal explants

J. Robert Bostwick, Stanley H. Appel, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    A central cholinergic trophic factor (C-CTF), previously reported in hippocampal extracts, enhances acetylcholine synthesis (ACh) and to a lesser extent choline acetyltransferase (ChAT) activity in cultured explants of the rat medial septal nucleus. Nerve growth factor (NGF) has been reported to enhance ChAT in several systems in vitro and in vivo, and clearly stimulates septal explants. At optimal concentrations of NGF and C-CTF, there is an additive effect on ChAT activity. The effects of NGF on ACh synthesis are minimal Antibodies to NGF block effects of added NGF but have no effects on C-CTF activity. The ability of C-CTF to enhance ACh synthesis appears related to its ability to enhance the acetylation of the choline that has been taken up by a sodium dependent, high affinity transport system. Thus, actions of NGF and C-CTF appear qualitatively and quantitatively distinct, yet both can influence the cholinergic activity of the developing medial septal nucleus.

    Original languageEnglish (US)
    Pages (from-to)92-98
    Number of pages7
    JournalBrain Research
    Volume422
    Issue number1
    DOIs
    StatePublished - Sep 29 1987

    Fingerprint

    Nerve Growth Factor
    Cholinergic Agents
    Choline O-Acetyltransferase
    Acetylcholine
    Septal Nuclei
    Acetylation
    Choline
    Sodium
    Antibodies

    Keywords

    • Cholinergic development
    • Nerve growth factor
    • Neurotrophic factor
    • Septal explant culture

    ASJC Scopus subject areas

    • Developmental Biology
    • Molecular Biology
    • Clinical Neurology
    • Neuroscience(all)

    Cite this

    Distinct influences of nerve growth factor and a central cholinergic trophic factor on medial septal explants. / Bostwick, J. Robert; Appel, Stanley H.; Perez-Polo, J. Regino.

    In: Brain Research, Vol. 422, No. 1, 29.09.1987, p. 92-98.

    Research output: Contribution to journalArticle

    Bostwick, J. Robert ; Appel, Stanley H. ; Perez-Polo, J. Regino. / Distinct influences of nerve growth factor and a central cholinergic trophic factor on medial septal explants. In: Brain Research. 1987 ; Vol. 422, No. 1. pp. 92-98.
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