TY - JOUR
T1 - Distinct patterns of expression of two VAMP genes within the rat brain
AU - Trimble, William S.
AU - Gray, Thackery S.
AU - Elferink, Lisa A.
AU - Wilson, Michael C.
AU - Scheller, Richard H.
PY - 1990
Y1 - 1990
N2 - VAMPs are synaptic vesicle-specific proteins composed of a carboxy-terminal Hydrophobic membrane anchor and an approximately 100 amino acid domain oriented towards the cytoplasm. In rat, two 77% homologous VAMP genes are expressed in the CMS. To precisely localize the neurons expressing these 2 forms of VAMP, we have used RNA blotting and in situ hybridization histochemistry with RNA probes specific for the 3′ untranslated regions of the transcripts. These experiments revealed that the 2 genes are expressed in distict, but slightly overlapping, patterns in the rat brain. VAMP-1 expression is localized to a limited number of nuclei, particularly those involved in modulating somatomotor functions, while VAMP-2 expression is more ubiquitous, being found in nuclei associated with autonomic, sensory, and integrative roles. These data suggest that the specific structural features of individual VAMPs may play an important role in synaptic vesicle metabolism.
AB - VAMPs are synaptic vesicle-specific proteins composed of a carboxy-terminal Hydrophobic membrane anchor and an approximately 100 amino acid domain oriented towards the cytoplasm. In rat, two 77% homologous VAMP genes are expressed in the CMS. To precisely localize the neurons expressing these 2 forms of VAMP, we have used RNA blotting and in situ hybridization histochemistry with RNA probes specific for the 3′ untranslated regions of the transcripts. These experiments revealed that the 2 genes are expressed in distict, but slightly overlapping, patterns in the rat brain. VAMP-1 expression is localized to a limited number of nuclei, particularly those involved in modulating somatomotor functions, while VAMP-2 expression is more ubiquitous, being found in nuclei associated with autonomic, sensory, and integrative roles. These data suggest that the specific structural features of individual VAMPs may play an important role in synaptic vesicle metabolism.
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U2 - 10.1523/jneurosci.10-04-01380.1990
DO - 10.1523/jneurosci.10-04-01380.1990
M3 - Article
C2 - 2329380
AN - SCOPUS:0025410534
SN - 0270-6474
VL - 10
SP - 1380
EP - 1387
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 4
ER -