Distinction of pulmonary small cell carcinoma from poorly differentiated squamous cell carcinoma

An immunohistochemical approach

Hong Zhang, Jing Liu, Philip T. Cagle, Timothy C. Allen, Alvaro C. Laga, Dani S. Zander

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Accurate morphologic distinction between small cell carcinoma and poorly differentiated squamous cell carcinoma has critical therapeutic significance, but can be limited by crush artifact, tumor necrosis, limited tumor representation, and overlapping morphologic features. We evaluated a panel of antibodies for their efficacy in distinguishing between these neoplasms. Formalin-fixed paraffin-embedded tissue sections of small cell carcinomas and poorly differentiated squamous cell carcinomas underwent immunohistochemical staining with antibodies to thyroid transcription factor-1, p63, high molecular weight keratin, and p16(INK4A). Of 28 small cell carcinomas, 26 (93%) small cell carcinomas showed diffuse moderate or strong staining for thyroid transcription factor-1 with no staining for high molecular weight keratin and p63. In contrast, 27/28 (96%) poorly differentiated squamous cell carcinomas manifested opposite immunoreactivities, with diffuse moderate or strong staining for high molecular weight keratin and p63, and no or minimal staining for thyroid transcription factor-1. In two additional cases originally interpreted as small cell carcinoma, high molecular weight keratin highlighted small numbers of neoplastic large cells, leading to reclassification as combined small cell and non-small cell carcinomas. p16(INK4A) expression varied widely in poorly differentiated squamous cell carcinomas, but was consistently moderate or strong and diffuse in small cell carcinomas, and proved helpful in the two thyroid transcription factor-1-negative small cell carcinomas. This study demonstrates that a panel consisting of antibodies to thyroid transcription factor-1, p63, high molecular weight keratin, and p16(INK4A) is highly effective for distinguishing between small cell carcinoma and poorly differentiated squamous cell carcinoma. This panel also facilitates diagnosis of combined small cell and non-small cell carcinomas.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalModern Pathology
Volume18
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Small Cell Carcinoma
Squamous Cell Carcinoma
Keratins
Lung
Cyclin-Dependent Kinase Inhibitor p16
Molecular Weight
Staining and Labeling
Antibodies
Carcinoma
Neoplasms
Paraffin
Artifacts
Formaldehyde
Necrosis
thyroid nuclear factor 1

Keywords

  • Immunohistochemistry
  • Lung
  • Small cell carcinoma
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Distinction of pulmonary small cell carcinoma from poorly differentiated squamous cell carcinoma : An immunohistochemical approach. / Zhang, Hong; Liu, Jing; Cagle, Philip T.; Allen, Timothy C.; Laga, Alvaro C.; Zander, Dani S.

In: Modern Pathology, Vol. 18, No. 1, 01.2005, p. 111-118.

Research output: Contribution to journalArticle

Zhang, Hong ; Liu, Jing ; Cagle, Philip T. ; Allen, Timothy C. ; Laga, Alvaro C. ; Zander, Dani S. / Distinction of pulmonary small cell carcinoma from poorly differentiated squamous cell carcinoma : An immunohistochemical approach. In: Modern Pathology. 2005 ; Vol. 18, No. 1. pp. 111-118.
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