Distinctive core histone post-translational modification patterns in Arabidopsis thaliana

Kangling Zhang, Vaniyambadi V. Sridhar, Jianhua Zhu, Avnish Kapoor, Jian Kang Zhu

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation of gene expression from chromatin. Studies in mammals and yeast have found conserved modifications at some residues of histories as well as non-conserved modifications at some other sites. Although plants have been excellent systems to study epigenetic regulation, and histone modifications are known to play critical roles, the histone modification sites and patterns in plants are poorly defined. In the present study we have used mass spectrometry in combination with high performance liquid chromatography (HPLC) separation and phospho-peptide enrichment to identify histone modification sites in the reference plant, Arabidopsis thaliana. We found not only modifications at many sites that are conserved in mammalian and yeast cells, but also modifications at many sites that are unique to plants, These unique modifications include H4 K20 acetylation (in contrast to H4 K20 methylation in non-plant systems), H2B K6, K11, K27 and K32 acetylation, S15 phosphorylation and K143 ubiquitination, and H2A K144 acetylation and S129, S141 and S145 phosphorylation, and H2A.X S138 phosphorylation. In addition, we found that lysine 79 of H3 which is highly conserved and modified by methylation and plays important roles in telomeric silencing in non-plant systems, is not modified in Arabidopsis. These results suggest distinctive histone modification patterns in plants and provide an invaluable foundation for future studies on histone modifications in plants.

Original languageEnglish (US)
Article numbere1210
JournalPLoS One
Volume2
Issue number11
DOIs
StatePublished - Nov 21 2007
Externally publishedYes

Fingerprint

Histone Code
post-translational modification
Post Translational Protein Processing
Arabidopsis
histones
Histones
Arabidopsis thaliana
Acetylation
Phosphorylation
acetylation
phosphorylation
Methylation
Epigenomics
epigenetics
methylation
Yeast
S 145
Yeasts
yeasts
Mammals

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Distinctive core histone post-translational modification patterns in Arabidopsis thaliana. / Zhang, Kangling; Sridhar, Vaniyambadi V.; Zhu, Jianhua; Kapoor, Avnish; Zhu, Jian Kang.

In: PLoS One, Vol. 2, No. 11, e1210, 21.11.2007.

Research output: Contribution to journalArticle

Zhang, Kangling ; Sridhar, Vaniyambadi V. ; Zhu, Jianhua ; Kapoor, Avnish ; Zhu, Jian Kang. / Distinctive core histone post-translational modification patterns in Arabidopsis thaliana. In: PLoS One. 2007 ; Vol. 2, No. 11.
@article{aadd9a8a03f74ea39e895aed840298c1,
title = "Distinctive core histone post-translational modification patterns in Arabidopsis thaliana",
abstract = "Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation of gene expression from chromatin. Studies in mammals and yeast have found conserved modifications at some residues of histories as well as non-conserved modifications at some other sites. Although plants have been excellent systems to study epigenetic regulation, and histone modifications are known to play critical roles, the histone modification sites and patterns in plants are poorly defined. In the present study we have used mass spectrometry in combination with high performance liquid chromatography (HPLC) separation and phospho-peptide enrichment to identify histone modification sites in the reference plant, Arabidopsis thaliana. We found not only modifications at many sites that are conserved in mammalian and yeast cells, but also modifications at many sites that are unique to plants, These unique modifications include H4 K20 acetylation (in contrast to H4 K20 methylation in non-plant systems), H2B K6, K11, K27 and K32 acetylation, S15 phosphorylation and K143 ubiquitination, and H2A K144 acetylation and S129, S141 and S145 phosphorylation, and H2A.X S138 phosphorylation. In addition, we found that lysine 79 of H3 which is highly conserved and modified by methylation and plays important roles in telomeric silencing in non-plant systems, is not modified in Arabidopsis. These results suggest distinctive histone modification patterns in plants and provide an invaluable foundation for future studies on histone modifications in plants.",
author = "Kangling Zhang and Sridhar, {Vaniyambadi V.} and Jianhua Zhu and Avnish Kapoor and Zhu, {Jian Kang}",
year = "2007",
month = "11",
day = "21",
doi = "10.1371/journal.pone.0001210",
language = "English (US)",
volume = "2",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Distinctive core histone post-translational modification patterns in Arabidopsis thaliana

AU - Zhang, Kangling

AU - Sridhar, Vaniyambadi V.

AU - Zhu, Jianhua

AU - Kapoor, Avnish

AU - Zhu, Jian Kang

PY - 2007/11/21

Y1 - 2007/11/21

N2 - Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation of gene expression from chromatin. Studies in mammals and yeast have found conserved modifications at some residues of histories as well as non-conserved modifications at some other sites. Although plants have been excellent systems to study epigenetic regulation, and histone modifications are known to play critical roles, the histone modification sites and patterns in plants are poorly defined. In the present study we have used mass spectrometry in combination with high performance liquid chromatography (HPLC) separation and phospho-peptide enrichment to identify histone modification sites in the reference plant, Arabidopsis thaliana. We found not only modifications at many sites that are conserved in mammalian and yeast cells, but also modifications at many sites that are unique to plants, These unique modifications include H4 K20 acetylation (in contrast to H4 K20 methylation in non-plant systems), H2B K6, K11, K27 and K32 acetylation, S15 phosphorylation and K143 ubiquitination, and H2A K144 acetylation and S129, S141 and S145 phosphorylation, and H2A.X S138 phosphorylation. In addition, we found that lysine 79 of H3 which is highly conserved and modified by methylation and plays important roles in telomeric silencing in non-plant systems, is not modified in Arabidopsis. These results suggest distinctive histone modification patterns in plants and provide an invaluable foundation for future studies on histone modifications in plants.

AB - Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation of gene expression from chromatin. Studies in mammals and yeast have found conserved modifications at some residues of histories as well as non-conserved modifications at some other sites. Although plants have been excellent systems to study epigenetic regulation, and histone modifications are known to play critical roles, the histone modification sites and patterns in plants are poorly defined. In the present study we have used mass spectrometry in combination with high performance liquid chromatography (HPLC) separation and phospho-peptide enrichment to identify histone modification sites in the reference plant, Arabidopsis thaliana. We found not only modifications at many sites that are conserved in mammalian and yeast cells, but also modifications at many sites that are unique to plants, These unique modifications include H4 K20 acetylation (in contrast to H4 K20 methylation in non-plant systems), H2B K6, K11, K27 and K32 acetylation, S15 phosphorylation and K143 ubiquitination, and H2A K144 acetylation and S129, S141 and S145 phosphorylation, and H2A.X S138 phosphorylation. In addition, we found that lysine 79 of H3 which is highly conserved and modified by methylation and plays important roles in telomeric silencing in non-plant systems, is not modified in Arabidopsis. These results suggest distinctive histone modification patterns in plants and provide an invaluable foundation for future studies on histone modifications in plants.

UR - http://www.scopus.com/inward/record.url?scp=43249091345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43249091345&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0001210

DO - 10.1371/journal.pone.0001210

M3 - Article

VL - 2

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e1210

ER -