Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum - An in vitro anterograde tracing study

Pablo R. Brumovsky, Jun-Ho La, Gerald F. Gebhart

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods: Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by 'bulk' (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results: Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences: We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.

Original languageEnglish (US)
Pages (from-to)1494-1507
Number of pages14
JournalNeurogastroenterology and Motility
Volume26
Issue number10
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Tissue Distribution
Calcitonin Gene-Related Peptide
Peripheral Nerves
Axons
Submucous Plexus
Neurons
Myenteric Plexus
Nerve Fibers
Confocal Microscopy
Ganglia
Blood Vessels
Mucous Membrane
Muscles
biotinamide
In Vitro Techniques

Keywords

  • Anterograde
  • Biotinamide
  • Colorectum
  • in vitro tracing
  • Peripheral nerves
  • Primary afferents

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology
  • Medicine(all)

Cite this

Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum - An in vitro anterograde tracing study. / Brumovsky, Pablo R.; La, Jun-Ho; Gebhart, Gerald F.

In: Neurogastroenterology and Motility, Vol. 26, No. 10, 01.10.2014, p. 1494-1507.

Research output: Contribution to journalArticle

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