Distribution and survival of Escherichia coli translocating from the intestine after thermal injury

J. Wesley Alexander, Luca Gianotti, Tonyia Eaves-Pyles, Mark A. Carey, George F. Babcock

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The present investigation was performed to study the kinetics of tissue distribution and deposition of Escherichia colt and endotoxin translocating from the intestine after thermal injury. Escherichia coli was grown in the presence of 14C glucose and both labeled bacteria and endotoxin prepared from the labeled bacteria were used as translocation probes. Escherichia coli (108 to 1010 bacteria) and E. coli endotoxin (100 μg per animal) were gavaged into the stomach immediately before a 30% burn injury was inflicted in mice. Animals were killed 1, 4, and 24 hours after burn injury. Translocation occurred extensively within 1 hour after burn injury. Expressed as amount of radioactivity per gram of tissue, translocation was greatest in the mesenteric lymph node (MLN) followed by spleen, lung, and liver. Translocation of endotoxin was similar to translocation of intact bacteria, with the exception that less radioactivity could be found in the peritoneal cavity and more in the liver. Both intact E. coli and endotoxin translocated directly through the intact bowel wall. Killing of bacteria was greatest in the MLN and spleen, approximating 95% to more than 99% of translocating bacteria. Killing efficiency was lowest in the lungs. It is concluded that estimation of translocation by viable bacterial counts in tissues grossly underestimates the extent of translocation of bacteria and ignores the extent of translocation of endotoxin. Translocation of en-dotoxin may have biologic significance that is independent of and in addition to translocation of intact bacteria.

Original languageEnglish (US)
Pages (from-to)558-566
Number of pages9
JournalAnnals of Surgery
Volume213
Issue number6
StatePublished - Jun 1991
Externally publishedYes

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Intestines
Hot Temperature
Escherichia coli
Bacteria
Wounds and Injuries
Endotoxins
Radioactivity
Spleen
Lymph Nodes
Escherichia
Lung
Bacterial Load
Liver
Peritoneal Cavity
Tissue Distribution
Stomach
Glucose

ASJC Scopus subject areas

  • Surgery

Cite this

Alexander, J. W., Gianotti, L., Eaves-Pyles, T., Carey, M. A., & Babcock, G. F. (1991). Distribution and survival of Escherichia coli translocating from the intestine after thermal injury. Annals of Surgery, 213(6), 558-566.

Distribution and survival of Escherichia coli translocating from the intestine after thermal injury. / Alexander, J. Wesley; Gianotti, Luca; Eaves-Pyles, Tonyia; Carey, Mark A.; Babcock, George F.

In: Annals of Surgery, Vol. 213, No. 6, 06.1991, p. 558-566.

Research output: Contribution to journalArticle

Alexander, JW, Gianotti, L, Eaves-Pyles, T, Carey, MA & Babcock, GF 1991, 'Distribution and survival of Escherichia coli translocating from the intestine after thermal injury', Annals of Surgery, vol. 213, no. 6, pp. 558-566.
Alexander, J. Wesley ; Gianotti, Luca ; Eaves-Pyles, Tonyia ; Carey, Mark A. ; Babcock, George F. / Distribution and survival of Escherichia coli translocating from the intestine after thermal injury. In: Annals of Surgery. 1991 ; Vol. 213, No. 6. pp. 558-566.
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