Distribution of bovine herpesvirus type 5 DNA in the central nervous systems of latently, experimentally infected calves

Fernanda Silveira Flôres Vogel, Luizinho Caron, Eduardo Furtado Flores, Rudi Weiblen, Evandro Reinoldo Winkelmann, Sandra Vanderli Mayer, Reginaldo Gaspar Bastos

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Bovine herpesvirus type 5 (BHV-5) is an alphaherpesvirus associated with meningoencephalitis, a disease highly prevalent in South America. In this study, we investigated the distribution of BHV-5 DNA in the brains of latently, experimentally infected calves by using a PCR for the glycoprotein B gene. Twelve calves inoculated intranasally with a Brazilian BHV-5 isolate were divided into two groups: group A calves (n = 4) were euthanized 55 days postinoculation (p.i.) for tissue collection; group B calves (n = 8) were submitted to dexamethasone administration at day 60 p.i. for reactivation of latent infection and were euthanized 50 days later. Latent infection was reactivated in all group B calves, as demonstrated by virus isolation from nasal secretions and/or seroconversion. Three calves developed neurological disease and died or were euthanized in extremis. For group A calves, viral DNA was consistently detected in the trigeminal ganglia (4/4), midbrain (4/4), thalamus (4/4), and olfactory cortex (4/4) and less frequently in the pons (3/4), cerebellum (3/4), anterior cerebral cortex (2/4), and olfactory bulb (2/4). For calves previously submitted to reactivation (group B), viral DNA was detected with roughly the same frequency in the same areas as for the group A calves. In addition, viral DNA was detected in the posterior (5/5) and dorso-lateral cortex (3/5). All DNA-positive tissues were negative for infectivity and viral antigens. These results demonstrated that latent BHV-5 DNA is present in several areas of the brain during latent infection and that virus reactivation may result in the establishment of latent infection in additional sites of the brain.

Original languageEnglish (US)
Pages (from-to)4512-4520
Number of pages9
JournalJournal of Clinical Microbiology
Volume41
Issue number10
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Bovine Herpesvirus 5
Viral DNA
Central Nervous System
DNA
Brain
Infection
Trigeminal Ganglion
Meningoencephalitis
Pons
Viral Antigens
South America
Olfactory Bulb
Virus Diseases
Mesencephalon
Thalamus
Nose
Cerebral Cortex
Cerebellum
Dexamethasone
Glycoproteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Vogel, F. S. F., Caron, L., Flores, E. F., Weiblen, R., Winkelmann, E. R., Mayer, S. V., & Bastos, R. G. (2003). Distribution of bovine herpesvirus type 5 DNA in the central nervous systems of latently, experimentally infected calves. Journal of Clinical Microbiology, 41(10), 4512-4520. https://doi.org/10.1128/JCM.41.10.4512-4520.2003

Distribution of bovine herpesvirus type 5 DNA in the central nervous systems of latently, experimentally infected calves. / Vogel, Fernanda Silveira Flôres; Caron, Luizinho; Flores, Eduardo Furtado; Weiblen, Rudi; Winkelmann, Evandro Reinoldo; Mayer, Sandra Vanderli; Bastos, Reginaldo Gaspar.

In: Journal of Clinical Microbiology, Vol. 41, No. 10, 01.10.2003, p. 4512-4520.

Research output: Contribution to journalArticle

Vogel, Fernanda Silveira Flôres ; Caron, Luizinho ; Flores, Eduardo Furtado ; Weiblen, Rudi ; Winkelmann, Evandro Reinoldo ; Mayer, Sandra Vanderli ; Bastos, Reginaldo Gaspar. / Distribution of bovine herpesvirus type 5 DNA in the central nervous systems of latently, experimentally infected calves. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 10. pp. 4512-4520.
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