Distribution of histochemically reactive zinc in the forebrain of the rat

C. J. Frederickson, B. A. Rampy, S. Reamy-Rampy, G. A. Howell

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The major cytoarchitectonic regions of the rat brain that stain with the Timm-Danscher metal stain were tested with the flourescent probe for zinc, 6-methoxy 8-para toluene sulfonamide quinoline (TSQ). Throughout most of the striatum, cerebral cortex and limbic system, the diffuse, even neuropil staining produced by the Timm-Danscher method was mirrored by comparable fluorescence in TSQ-stained sections. Blockade of the TSQ fluorescence by prior treatment with sulphide indicated that the Timm Danscher and the TSQ procedures both labeled the same pool of endogenous metal, which is inferred to be the zinc that is in axonal boutons. It is concluded that the Timm Danscher staining generally indicates zinc-containing axonal boutons. The distribution of the zinc-containing axonal boutons throughout the forebrain is described.

Original languageEnglish (US)
Pages (from-to)521-530
Number of pages10
JournalJournal of Chemical Neuroanatomy
Volume5
Issue number6
DOIs
StatePublished - 1992

Fingerprint

Sulfonamides
Toluene
Prosencephalon
Zinc
Coloring Agents
Fluorescence
Metals
Staining and Labeling
Limbic System
Neuropil
Sulfides
Cerebral Cortex
quinoline
Brain

Keywords

  • Axonal boutons
  • Timm-Danscher staining
  • TSQ
  • Vesicles

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Distribution of histochemically reactive zinc in the forebrain of the rat. / Frederickson, C. J.; Rampy, B. A.; Reamy-Rampy, S.; Howell, G. A.

In: Journal of Chemical Neuroanatomy, Vol. 5, No. 6, 1992, p. 521-530.

Research output: Contribution to journalArticle

Frederickson, C. J. ; Rampy, B. A. ; Reamy-Rampy, S. ; Howell, G. A. / Distribution of histochemically reactive zinc in the forebrain of the rat. In: Journal of Chemical Neuroanatomy. 1992 ; Vol. 5, No. 6. pp. 521-530.
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