Distribution of lysosomal hydrolases in human and bovine ocular tissues

Lynn A. Hawkins, Hari S. Garg, Yogesh C. Awasthi, Satish Srivastava

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have studied the distribution of the lysosoma1 sphingolipid hydrolases βglucosaminidase, βgalactosaminidase, βgalactosidase, α and βglucosidases, and αmannosidase in the bovine and human ocular tissues, choroid, cornea, lens, retina, and sclera using synthetic substrates in the form of the 4-methylumbelliferyl derivatives of the corresponding glycosides. As compared to the bovine ocular tissues, the human ocular tissues possessed higher levels of all the enzyme activities examined with the exception of βglucosidase in the cornea. Bovine retina contained higher levels of fi-hexosaminidases, βgalactosidase, and a-glucosidase than the other bovine ocular tissues. In contrast to the retina, which is primarily a neural tissue, human and bovine lens have minimal or trace levels of all the lysosomal hydrolases examined. Human and bovine retina, cornea, sclera, and choroid possess enzyme activities which are higher than the lens. This would indicate a slow turnover of glycosphingolipids in lens tissue as compared to the other ocular tissues.

Original languageEnglish (US)
Pages (from-to)497-500
Number of pages4
JournalCurrent Eye Research
Volume1
Issue number9
DOIs
StatePublished - 1981

Fingerprint

Hydrolases
Glucosidases
Hexosaminidases
Lenses
Retina
Galactosidases
Cornea
Sclera
Choroid
Mannosidases
Glycosphingolipids
Sphingolipids
Enzymes
Glycosides

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Hawkins, L. A., Garg, H. S., Awasthi, Y. C., & Srivastava, S. (1981). Distribution of lysosomal hydrolases in human and bovine ocular tissues. Current Eye Research, 1(9), 497-500. https://doi.org/10.3109/02713688109069174

Distribution of lysosomal hydrolases in human and bovine ocular tissues. / Hawkins, Lynn A.; Garg, Hari S.; Awasthi, Yogesh C.; Srivastava, Satish.

In: Current Eye Research, Vol. 1, No. 9, 1981, p. 497-500.

Research output: Contribution to journalArticle

Hawkins, LA, Garg, HS, Awasthi, YC & Srivastava, S 1981, 'Distribution of lysosomal hydrolases in human and bovine ocular tissues', Current Eye Research, vol. 1, no. 9, pp. 497-500. https://doi.org/10.3109/02713688109069174
Hawkins, Lynn A. ; Garg, Hari S. ; Awasthi, Yogesh C. ; Srivastava, Satish. / Distribution of lysosomal hydrolases in human and bovine ocular tissues. In: Current Eye Research. 1981 ; Vol. 1, No. 9. pp. 497-500.
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