Diverging mechanisms of activation of chemokine receptors revealed by novel chemokine agonists

Jose Sarmiento, Christie Shumate, Katsutoshi Suetomi, Aishwarya Ravindran, León Villegas, Krishna Rajarathnam, Javier Navarro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

CXCL8/interleukin-8 is a pro-inflammatory chemokine that triggers pleiotropic responses, including inflammation, angiogenesis, wound healing and tumorigenesis. We engineered the first selective CXCR1 agonists on the basis of residue substitutions in the conserved ELR triad and CXC motif of CXCL8. Our data reveal that the molecular mechanisms of activation of CXCR1 and CXCR2 are distinct: the N-loop of CXCL8 is the major determinant for CXCR1 activation, whereas the N-terminus of CXCL8 (ELR and CXC) is essential for CXCR2 activation. We also found that activation of CXCR1 cross-desensitized CXCR2 responses in human neutrophils co-expressing both receptors, indicating that these novel CXCR1 agonists represent a new class of anti-inflammatory agents. Further, these selective CXCR1 agonists will aid at elucidating the functional significance of CXCR1 in vivo under pathophysiological conditions.

Original languageEnglish (US)
Article numbere27967
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 9 2011

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Chemokine Receptors
chemokines
Interleukin-8
Chemokines
Wound Healing
agonists
Carcinogenesis
Neutrophils
Anti-Inflammatory Agents
Chemical activation
Inflammation
anti-inflammatory agents
interleukin-8
angiogenesis
tissue repair
carcinogenesis
neutrophils
inflammation
receptors
Substitution reactions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Diverging mechanisms of activation of chemokine receptors revealed by novel chemokine agonists. / Sarmiento, Jose; Shumate, Christie; Suetomi, Katsutoshi; Ravindran, Aishwarya; Villegas, León; Rajarathnam, Krishna; Navarro, Javier.

In: PLoS One, Vol. 6, No. 12, e27967, 09.12.2011.

Research output: Contribution to journalArticle

Sarmiento, Jose ; Shumate, Christie ; Suetomi, Katsutoshi ; Ravindran, Aishwarya ; Villegas, León ; Rajarathnam, Krishna ; Navarro, Javier. / Diverging mechanisms of activation of chemokine receptors revealed by novel chemokine agonists. In: PLoS One. 2011 ; Vol. 6, No. 12.
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