Diversity within Natural Populations of Eastern Equine Encephalomyelitis Virus

Scott Weaver, Liz Anne Bellew, Laurent Gousset, Patricia M. Repik, Thomas W. Scott, John J. Holland

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We evaluated genetic and phenotypic diversity within natural populations of the alphavirus, Eastern equine encephalomyelitis (EEE) virus. RNA fingerprinting revealed that most populations within infected hosts (unpassaged isolates) contained a consensus genotype along with minority genotypes differing in one to three T1-resistant oligonucleotides. Mutation frequencies appeared to be similar to those reported for other RNA viruses, suggesting that the slow rate of EEE virus evolution is not limited by fidelity of genome replication. Within a given year, genetic diversity was generally greater among geographically distant isolates than among those from the same transmission focus, suggesting that dispersal among EEE viruses in North America is not complete annually. Two of three bird isolates from Maryland and New York contained relatively distantly related genotypes, differing in 15-19 oligonucleotides. A 1985 mosquito isolate from Maryland contained stable, small plague variants which comprised the majority of that population. These small plaque variants differed by up to eight T1-resistant oligonucleotides when compared with their large plaque counterparts. Temperature sensitive virus was not detected in six unpassaged mosquito isolates from Maryland and New York.

Original languageEnglish (US)
Pages (from-to)700-709
Number of pages10
JournalVirology
Volume195
Issue number2
DOIs
StatePublished - Aug 1993
Externally publishedYes

Fingerprint

Eastern equine encephalitis virus
Oligonucleotides
Genotype
Culicidae
Population
Alphavirus
Plague
RNA Viruses
Mutation Rate
North America
Birds
Genome
RNA
Viruses
Temperature

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Weaver, S., Bellew, L. A., Gousset, L., Repik, P. M., Scott, T. W., & Holland, J. J. (1993). Diversity within Natural Populations of Eastern Equine Encephalomyelitis Virus. Virology, 195(2), 700-709. https://doi.org/10.1006/viro.1993.1421

Diversity within Natural Populations of Eastern Equine Encephalomyelitis Virus. / Weaver, Scott; Bellew, Liz Anne; Gousset, Laurent; Repik, Patricia M.; Scott, Thomas W.; Holland, John J.

In: Virology, Vol. 195, No. 2, 08.1993, p. 700-709.

Research output: Contribution to journalArticle

Weaver, S, Bellew, LA, Gousset, L, Repik, PM, Scott, TW & Holland, JJ 1993, 'Diversity within Natural Populations of Eastern Equine Encephalomyelitis Virus', Virology, vol. 195, no. 2, pp. 700-709. https://doi.org/10.1006/viro.1993.1421
Weaver, Scott ; Bellew, Liz Anne ; Gousset, Laurent ; Repik, Patricia M. ; Scott, Thomas W. ; Holland, John J. / Diversity within Natural Populations of Eastern Equine Encephalomyelitis Virus. In: Virology. 1993 ; Vol. 195, No. 2. pp. 700-709.
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