DNA-associated click chemistry

Mohammad Mojibul Haque; Xiaohua Peng

doi:10.1007/s11426-013-5035-1

DNA-associated click chemistry

Mohammad Mojibul Haque
, Xiaohua Peng

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

This review highlights the most recent advances in click chemistry associated with DNA. Cu[I]-catalyzed azides-alkynes Huisgen cycloadditions (CuAAC) and a strain-promoted alkyne-azide cycloaddition (SPAAC) are two popular click reactions that have great impact in DNA science. The simplicity, versatility, orthogonality, and high efficiency of click reaction along with a stable triazole product have been instrumental for the successful application of this reaction in the field of nucleic acid chemistry. CuAAC and SPAAC reactions have been widely used for DNA modification, including DNA labeling, metallization, conjugation, cross-linking, and ligation. Modified oligodeoxynucleotides obtained from click reaction have been extensively applied in the fields of drug discovery, nanotechnology, bio-conjugation, and material sciences, among others. The most recent advances in the synthesis and applications of clickable DNAs are discussed in detail in this article.

Original language	English (US)
Pages (from-to)	215-231
Number of pages	17
Journal	Science China Chemistry
Volume	57
Issue number	2
DOIs	https://doi.org/10.1007/s11426-013-5035-1
State	Published - Feb 2014
Externally published	Yes

Keywords

"click" chemistry
DNA functionalization
DNA ligation and cross-linking
oligodeoxynucleotides

ASJC Scopus subject areas

General Chemistry

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10.1007/s11426-013-5035-1

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title = "DNA-associated click chemistry",

abstract = "This review highlights the most recent advances in click chemistry associated with DNA. Cu[I]-catalyzed azides-alkynes Huisgen cycloadditions (CuAAC) and a strain-promoted alkyne-azide cycloaddition (SPAAC) are two popular click reactions that have great impact in DNA science. The simplicity, versatility, orthogonality, and high efficiency of click reaction along with a stable triazole product have been instrumental for the successful application of this reaction in the field of nucleic acid chemistry. CuAAC and SPAAC reactions have been widely used for DNA modification, including DNA labeling, metallization, conjugation, cross-linking, and ligation. Modified oligodeoxynucleotides obtained from click reaction have been extensively applied in the fields of drug discovery, nanotechnology, bio-conjugation, and material sciences, among others. The most recent advances in the synthesis and applications of clickable DNAs are discussed in detail in this article.",

keywords = "{"}click{"} chemistry, DNA functionalization, DNA ligation and cross-linking, oligodeoxynucleotides",

author = "Haque, \{Mohammad Mojibul\} and Xiaohua Peng",

note = "Funding Information: We are grateful for the financial support from the UWM Research Growth Initiative (RGI101X234), the Greater Milwaukee Foundation (Shaw Scientist Award), and the National Cancer Institute (1R15CA152914-01). We thank all of our collaborators and co-workers whose names appear in the reference list.",

year = "2014",

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doi = "10.1007/s11426-013-5035-1",

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AU - Haque, Mohammad Mojibul

AU - Peng, Xiaohua

N1 - Funding Information: We are grateful for the financial support from the UWM Research Growth Initiative (RGI101X234), the Greater Milwaukee Foundation (Shaw Scientist Award), and the National Cancer Institute (1R15CA152914-01). We thank all of our collaborators and co-workers whose names appear in the reference list.

PY - 2014/2

Y1 - 2014/2

N2 - This review highlights the most recent advances in click chemistry associated with DNA. Cu[I]-catalyzed azides-alkynes Huisgen cycloadditions (CuAAC) and a strain-promoted alkyne-azide cycloaddition (SPAAC) are two popular click reactions that have great impact in DNA science. The simplicity, versatility, orthogonality, and high efficiency of click reaction along with a stable triazole product have been instrumental for the successful application of this reaction in the field of nucleic acid chemistry. CuAAC and SPAAC reactions have been widely used for DNA modification, including DNA labeling, metallization, conjugation, cross-linking, and ligation. Modified oligodeoxynucleotides obtained from click reaction have been extensively applied in the fields of drug discovery, nanotechnology, bio-conjugation, and material sciences, among others. The most recent advances in the synthesis and applications of clickable DNAs are discussed in detail in this article.

AB - This review highlights the most recent advances in click chemistry associated with DNA. Cu[I]-catalyzed azides-alkynes Huisgen cycloadditions (CuAAC) and a strain-promoted alkyne-azide cycloaddition (SPAAC) are two popular click reactions that have great impact in DNA science. The simplicity, versatility, orthogonality, and high efficiency of click reaction along with a stable triazole product have been instrumental for the successful application of this reaction in the field of nucleic acid chemistry. CuAAC and SPAAC reactions have been widely used for DNA modification, including DNA labeling, metallization, conjugation, cross-linking, and ligation. Modified oligodeoxynucleotides obtained from click reaction have been extensively applied in the fields of drug discovery, nanotechnology, bio-conjugation, and material sciences, among others. The most recent advances in the synthesis and applications of clickable DNAs are discussed in detail in this article.

KW - "click" chemistry

KW - DNA functionalization

KW - DNA ligation and cross-linking

KW - oligodeoxynucleotides

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DNA-associated click chemistry

Abstract

Keywords

ASJC Scopus subject areas

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