DNA barcoding the commercial Chinese caterpillar fungus

Li Xiang, Jingyuan Song, Tianyi Xin, Yingjie Zhu, Linchun Shi, Xiaolan Xu, Xiaohui Pang, Hui Yao, Wenjia Li, Shilin Chen

Research output: Contribution to journalLetter

36 Scopus citations

Abstract

Chinese caterpillar fungus (Ophiocordyceps sinensis) has been widely used as tonic in Asian medicine. Considering its curative effect and high cost, various counterfeit versions of O. sinensis have been introduced and are commercially available. These counterfeits have morphological characteristics that are difficult to distinguish based on morphology alone, thereby causing confusion and threatening its safe use. In this study, internal transcribed spacer (ITS) sequences as a DNA barcode were analyzed and assessed for rapid and accurate identification of 131 O. sinensis samples and 12 common counterfeits and closely related species. Results showed that sufficient ITS sequence differences, also known as 'barcode gaps', existed to distinguish between O. sinensis and counterfeit species. ITS sequence correctly identified 100% of the samples at the species and genus level using the Basic Local Alignment Search Tool 1 and the nearest distance method. Furthermore, O. sinensis, counterfeits, and closely related species can be successfully identified using tree-based methods including maximum parsimony, neighbor-joining, and maximum likelihood analysis. These results indicated that DNA barcoding could be used as a fast and accurate identification method to distinguish O. sinensis from counterfeits and closely related species to ensure its safe use.

Original languageEnglish (US)
Pages (from-to)156-162
Number of pages7
JournalFEMS Microbiology Letters
Volume347
Issue number2
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

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Keywords

  • Counterfeit
  • DNA barcode
  • Internal transcribed spacer
  • Ophiocordyceps sinensis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Xiang, L., Song, J., Xin, T., Zhu, Y., Shi, L., Xu, X., Pang, X., Yao, H., Li, W., & Chen, S. (2013). DNA barcoding the commercial Chinese caterpillar fungus. FEMS Microbiology Letters, 347(2), 156-162. https://doi.org/10.1111/1574-6968.12233