DNA microarray analysis of the contused spinal cord: Effect of NMDA receptor inhibition

O. Nesic, N. M. Svrakic, G. Y. Xu, D. McAdoo, K. N. Westlund, C. E. Hulsebosch, Zeiming Ye, A. Galante, P. Soteropoulos, P. Tolias, W. Young, R. P. Hart, J. R. Perez-Polo

    Research output: Contribution to journalArticle

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    Abstract

    Spinal cord injury (SCI)-induced neurodegeneration leads to irreversible and devastating motor and sensory dysfunction. Post-traumatic outcomes are determined by events occurring during the first 24 hours after SCI. An increase in extracellular glutamate concentration to neurotoxic levels is one of the earliest events after SCI. We used Affymetrix DNA oligonucleotide microarrays (with 1,322 DNA probes) analysis to measure gene expression in order to test the hypothesis that SCI-induced N-methyl-D-aspartate (NMDA) receptor activation triggers significant postinjury transcriptional changes. Here we report that SCI, 1 hour after trauma, induced change in mRNA levels of 165 genes and expression sequence tags (ESTs). SCI affected mRNA levels of those genes that regulate predominantly transcription factors, inflammation, cell survival, and membrane excitability. We also report that NMDA receptor inhibition (with -(+)-5-methyl-10,11 -dihydro-5H-dibenzo[a,d]-cyclohepten-5, 10-imine hydrogen maleate [MK-801]) reversed the effect of SCI on about 50% of the SCI-affected mRNAs. Especially interesting is the finding that NMDA receptor activation participates in the up-regulation of inflammatory factors. Therefore, SCI-induced NMDA receptor activation is one of the dominant, early signals after trauma that leads to changes in mRNA levels of a number of genes relevant to recovery processes. The majority of MK-801 effects on the SCI-induced mRNA changes reported here are novel. Additionally, we found that the MK-801 treatment also changed the mRNA levels of 168 genes and ESTs that had not been affected by SCI alone, and that some of their gene products could have harmful effects on SCI outcome.

    Original languageEnglish (US)
    Pages (from-to)406-423
    Number of pages18
    JournalJournal of Neuroscience Research
    Volume68
    Issue number4
    DOIs
    StatePublished - May 15 2002

    Fingerprint

    Contusions
    Microarray Analysis
    Oligonucleotide Array Sequence Analysis
    N-Methyl-D-Aspartate Receptors
    Spinal Cord Injuries
    Spinal Cord
    Messenger RNA
    Dizocilpine Maleate
    Gene Expression
    Genes
    Imines
    DNA Probes
    Wounds and Injuries
    Glutamic Acid
    Cell Survival
    Transcription Factors
    Up-Regulation
    Cell Membrane

    Keywords

    • Affymetrix
    • DNA microarray
    • Expression profile
    • MK-801
    • NMDA receptor
    • Spinal cord injury

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Nesic, O., Svrakic, N. M., Xu, G. Y., McAdoo, D., Westlund, K. N., Hulsebosch, C. E., ... Perez-Polo, J. R. (2002). DNA microarray analysis of the contused spinal cord: Effect of NMDA receptor inhibition. Journal of Neuroscience Research, 68(4), 406-423. https://doi.org/10.1002/jnr.10171

    DNA microarray analysis of the contused spinal cord : Effect of NMDA receptor inhibition. / Nesic, O.; Svrakic, N. M.; Xu, G. Y.; McAdoo, D.; Westlund, K. N.; Hulsebosch, C. E.; Ye, Zeiming; Galante, A.; Soteropoulos, P.; Tolias, P.; Young, W.; Hart, R. P.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 68, No. 4, 15.05.2002, p. 406-423.

    Research output: Contribution to journalArticle

    Nesic, O, Svrakic, NM, Xu, GY, McAdoo, D, Westlund, KN, Hulsebosch, CE, Ye, Z, Galante, A, Soteropoulos, P, Tolias, P, Young, W, Hart, RP & Perez-Polo, JR 2002, 'DNA microarray analysis of the contused spinal cord: Effect of NMDA receptor inhibition', Journal of Neuroscience Research, vol. 68, no. 4, pp. 406-423. https://doi.org/10.1002/jnr.10171
    Nesic O, Svrakic NM, Xu GY, McAdoo D, Westlund KN, Hulsebosch CE et al. DNA microarray analysis of the contused spinal cord: Effect of NMDA receptor inhibition. Journal of Neuroscience Research. 2002 May 15;68(4):406-423. https://doi.org/10.1002/jnr.10171
    Nesic, O. ; Svrakic, N. M. ; Xu, G. Y. ; McAdoo, D. ; Westlund, K. N. ; Hulsebosch, C. E. ; Ye, Zeiming ; Galante, A. ; Soteropoulos, P. ; Tolias, P. ; Young, W. ; Hart, R. P. ; Perez-Polo, J. R. / DNA microarray analysis of the contused spinal cord : Effect of NMDA receptor inhibition. In: Journal of Neuroscience Research. 2002 ; Vol. 68, No. 4. pp. 406-423.
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    AU - McAdoo, D.

    AU - Westlund, K. N.

    AU - Hulsebosch, C. E.

    AU - Ye, Zeiming

    AU - Galante, A.

    AU - Soteropoulos, P.

    AU - Tolias, P.

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