DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal, Deepak Nair, Robert Johnson, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

Original languageEnglish (US)
Pages (from-to)E7
JournalNature
Volume437
Issue number7057
DOIs
StatePublished - Sep 14 2005

ASJC Scopus subject areas

  • Medicine(all)
  • General

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