DNA polymerases

Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal, Deepak Nair, Robert Johnson, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

Original languageEnglish (US)
Pages (from-to)E7
JournalNature
Volume437
Issue number7057
DOIs
StatePublished - Sep 14 2005

Fingerprint

DNA-Directed DNA Polymerase
DNA Replication
Base Pairing
Electrons
Nucleotides
DNA

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

DNA polymerases : Hoogsteen base-pairing in DNA replication? (reply). / Aggarwal, Aneel; Nair, Deepak; Johnson, Robert; Prakash, Louise; Prakash, Satya.

In: Nature, Vol. 437, No. 7057, 14.09.2005, p. E7.

Research output: Contribution to journalArticle

Aggarwal, Aneel ; Nair, Deepak ; Johnson, Robert ; Prakash, Louise ; Prakash, Satya. / DNA polymerases : Hoogsteen base-pairing in DNA replication? (reply). In: Nature. 2005 ; Vol. 437, No. 7057. pp. E7.
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