Abstract
We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | E7 |
| Journal | Nature |
| Volume | 437 |
| Issue number | 7057 |
| DOIs | |
| State | Published - Sep 14 2005 |
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ASJC Scopus subject areas
- Medicine(all)
- General
Cite this
DNA polymerases : Hoogsteen base-pairing in DNA replication? (reply). / Aggarwal, Aneel; Nair, Deepak; Johnson, Robert; Prakash, Louise; Prakash, Satya.
In: Nature, Vol. 437, No. 7057, 14.09.2005, p. E7.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA polymerases
T2 - Hoogsteen base-pairing in DNA replication? (reply)
AU - Aggarwal, Aneel
AU - Nair, Deepak
AU - Johnson, Robert
AU - Prakash, Louise
AU - Prakash, Satya
PY - 2005/9/14
Y1 - 2005/9/14
N2 - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.
AB - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.
UR - http://www.scopus.com/inward/record.url?scp=84984761775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84984761775&partnerID=8YFLogxK
U2 - 10.1038/nature04200
DO - 10.1038/nature04200
M3 - Article
AN - SCOPUS:84984761775
VL - 437
SP - E7
JO - Nature
JF - Nature
SN - 0028-0836
IS - 7057
ER -