DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal; Deepak Nair; Robert Johnson; Louise Prakash; Satya Prakash

doi:10.1038/nature04200

DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Aneel Aggarwal, Deepak Nair, Robert Johnson, Louise Prakash, Satya Prakash

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

Original language	English (US)
Pages (from-to)	E7
Journal	Nature
Volume	437
Issue number	7057
DOIs	https://doi.org/10.1038/nature04200
State	Published - Sep 14 2005

ASJC Scopus subject areas

General

Access to Document

10.1038/nature04200

Cite this

@article{b236f73e7c3e4e73babd034845d1c77e,

title = "DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)",

abstract = "We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.",

author = "Aneel Aggarwal and Deepak Nair and Robert Johnson and Louise Prakash and Satya Prakash",

year = "2005",

month = sep,

day = "14",

doi = "10.1038/nature04200",

language = "English (US)",

volume = "437",

pages = "E7",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "7057",

}

TY - JOUR

T1 - DNA polymerases

T2 - Hoogsteen base-pairing in DNA replication? (reply)

AU - Aggarwal, Aneel

AU - Nair, Deepak

AU - Johnson, Robert

AU - Prakash, Louise

AU - Prakash, Satya

PY - 2005/9/14

Y1 - 2005/9/14

N2 - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

AB - We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation.

UR - http://www.scopus.com/inward/record.url?scp=84984761775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984761775&partnerID=8YFLogxK

U2 - 10.1038/nature04200

DO - 10.1038/nature04200

M3 - Article

AN - SCOPUS:84984761775

VL - 437

SP - E7

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7057

ER -

DNA polymerases: Hoogsteen base-pairing in DNA replication? (reply)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this