DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II

Sami N. Guzder, Hongfang Qiu, Christopher H. Sommers, Patrick Sung, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticlepeer-review

125 Scopus citations


THE RAD3 gene of Saccharomyces cerevisiae is required for excision repair of ultraviolet-damaged DNA and is essential for cell viability1. The RAD3-encoded protein shares a high degree of homology with the human ERCC2(XPD) gene product2. Mutations in XPD, besides causing the cancer-prone syndrome xeroderma pigmentosum, can also result in Cockayne's syndrome and trichothiodystrophy3. To investigate the role of RAD3 in viability, we examine here the effect of a recessive, temperature-sensitive (ts) conditional lethal mutation of the gene on transcription by RNA polymerase II. Upon transfer to the restrictive temperature, the rad3-ts mutant rapidly ceases growth and poly(A)+ RNA synthesis is inhibited drastically. Messenger RNA levels of all the genes examined, HIS3, TRP3, STE2, MET19, RAD23, CDC7, CDC9 and ACT1, decline rapidly upon loss of RAD3 activity. The synthesis of heat-shock-inducible HSP26 mRNA and galactose-inducible GAL7 and GAL10 mRNAs is also drastically inhibited in the rad3-ts mutant at the restrictive temperature. The RNA polymerase II transcriptional activity in extract from therad3-tsstrain is thermolabile, and this in vitro transcriptional defect can be fully corrected by the addition of homogeneous RAD3 protein. These findings indicate that RAD3 protein has a direct and essential role in RNA polymerase II transcription.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
Issue number6458
StatePublished - 1994

ASJC Scopus subject areas

  • General


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